By a News Reporter-Staff News Editor at Biotech Week -- Current study results on Enzymes and Coenzymes have been published. According to news reporting originating from Bron, France, by NewsRx correspondents, research stated, "APOA5 c.*158C >T (rs2266788), located in the 3' UTR, belongs to APOA5 haplotype 2 (APOA5*2), which is strongly associated with plasma triglyceride levels and modulates the occurrence of both moderate and severe hypertriglyceridemia. Individuals with APOA5*2 display reduced APOA5 expression at the posttranscriptional level."
Our news editors obtained a quote from the research from Hospices civils de Lyon, "However, the functionality of this haplotype remains unclear. We hypothesized that the hypertriglyceridemic effects of APOA5*2 could involve miRNA regulation in the APOA5 3' UTR. Bioinformatic studies have identified the creation of a potential miRNA binding site for liver-expressed miR-485-5p (MIRN485-5p) in the mutant APOA5 3' UTR with the c.*158C allele. In human embryonic kidney 293T (HEK293T) cells cotransfected with an APOA5 3' UTR luciferase reporter vector and a miR485-5p precursor, c.*158C allele expression was significantly decreased. Moreover, in HuH-7 cells endogenously expressing miR-485-5p, we observed that luciferase activity was significantly lower in the presence of the c.*158C allele than in the presence of the c.*158T allele, which was completely reversed by a miR-485-5p inhibitor. We demonstrated that the rare c.*158C APOA5 allele creates a functional target site for liver-expressed miR-485-5p."
According to the news editors, the research concluded: "Therefore, we propose that the well-documented hypertriglyceridemic effect of APOA5*2 involves an APOA5 posttranscriptional downregulation mediated by miR-485-5p."
For more information on this research see: An APOA5 3 ' UTR Variant Associated with Plasma Triglycerides Triggers APOA5 Downregulation by Creating a Functional miR-485-5p Binding Site. American Journal of Human Genetics, 2014;94(1):129-134. American Journal of Human Genetics can be contacted at: Cell Press, 600 Technology Square, 5TH Floor, Cambridge, MA 02139, USA. (Elsevier - www.elsevier.com; American Journal of Human Genetics - www.elsevier.com/wps/product/cws_home/713561)
The news editors report that additional information may be obtained by contacting C. Caussy, Hosp Civils Lyon, Center Biol & Pathol Est, Dept. of Biochim & Biol Mol, F-69677 Bron, France. Additional authors for this research include S. Charriere, C. Marcais, M. Di Filippo, A. Sassolas, M. Delay, V. Euthine, A. Jalabert, E. Lefai, S. Rome and P. Moulin (see also Enzymes and Coenzymes).
Keywords for this news article include: Bron, France, Europe, Enzymes and Coenzymes
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