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Research from Nencki Institute of Experimental Biology Yields New Findings on Biosensing

February 19, 2014

By a News Reporter-Staff News Editor at Biotech Week -- Investigators publish new report on Biosensing. According to news reporting originating from Warszawa, Poland, by NewsRx correspondents, research stated, "A genetically encoded Forster Resonance Energy Transfer (FRET)-based biosensor that continuously monitors matrix metalloproteinase 9 (MMP-9) activity was developed. MMP-9 is an extracellularly acting endopeptidase with a prominent role in development, learning and memory, cancer metastasis, and stroke."

Our news editors obtained a quote from the research from the Nencki Institute of Experimental Biology, "To assess the biological function of the protease, determining the precise kinetics and localization of MMP-9 activity is required. The nontoxic, genetically encoded FRET biosensor presented herein is anchored in the cellular membrane and thus provides an important advantage over currently employed probes. The biosensor allows the study of the proteolytic activity of MMP-9 with high temporal and subcellular resolution at the precise region of MMP-9 action on the cell. The applicability of the biosensor both in vitro and in living cells was demonstrated by ratiometrically analyzing the cleavage of the biosensor by a purified auto-activating mutant of MMP-9 and endogenously secreted protease in cultured tumor and neuronal cells."

According to the news editors, the research concluded: "The precise kinetics of endogenous MMP-9 activity was measured, which demonstrates in a straight-forward manner the applicability of the biosensor concept."

For more information on this research see: Genetically encoded FRET-based biosensor for imaging MMP-9 activity. Biomaterials, 2014;35(5):1402-10. (Elsevier -; Biomaterials -

The news editors report that additional information may be obtained by contacting M. Stawarski, Dept. of Molecular and Cellular Neurobiology, The Nencki Institute of Experimental Biology, ul Ludwika Pasteura 3, Warszawa 02-093, Poland. Additional authors for this research include I. Rutkowska-Wlodarczyk, A. Zeug, M. Bijata, H. Madej, L. Kaczmarek and J. Wlodarczyk (see also Biosensing).

Keywords for this news article include: Poland, Europe, Warszawa, Biosensing, Bioengineering, Bionanotechnology, Nanobiotechnology.

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Source: Biotech Week

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