By a News Reporter-Staff News Editor at Life Science Weekly -- Data detailed on DNA Research have been presented. According to news reporting originating from Leeds, United Kingdom, by NewsRx correspondents, research stated, "We report herein the development of a simple, sensitive colorimetric magnetic nanoparticle (MNP)-enzyme-based DNA sandwich assay that is suitable for simultaneous, label-free quantitation of two DNA targets down to 50 fM level. It can also effectively discriminate single-nucleotide polymorphisms (SNPs) in genes associated with human cancers (KRAS codon 12/13 SNPs)."
Our news editors obtained a quote from the research from the University of Leeds, "This assay uses a pair of specific DNA probes, one being covalently conjugated to an MNP for target capture and the other being linked to an enzyme for signal amplification, to sandwich a DNA target, allowing for convenient magnetic separation and subsequent efficient enzymatic signal amplification for high sensitivity. Careful optimization of the MNP surfaces and assay conditions greatly reduced the background, allowing for sensitive, specific detection of as little as 5 amol (50 fM in 100 mu L) of target DNA. Moreover, this sensor is robust, it can effectively discriminate cancer-specific SNPs against the wild-type noncancer target, and it works efficiently in 10% human serum. Furthermore, this sensor can simultaneously quantitate two different DNA targets by using two pairs of unique capture- and signal-DNA probes specific for each target."
According to the news editors, the research concluded: "This general, simple, and sensitive DNA sensor appears to be well-suited for a wide range of genetics-based biosensing and diagnostic applications."
For more information on this research see: Sensitive, Simultaneous Quantitation of Two Unlabeled DNA Targets Using a Magnetic Nanoparticle-Enzyme Sandwich Assay. Analytical Chemistry, 2013;85(19):9238-9244. Analytical Chemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Analytical Chemistry - www.pubs.acs.org/journal/ancham)
The news editors report that additional information may be obtained by contacting Y. Zhang, University of Leeds, St Jamess Univ Hosp, Leeds Inst Mol Med, Sect Pathol & Tumour Biol, Leeds LS9 7TF, W Yorkshire, United Kingdom. Additional authors for this research include C. Pilapong, Y. Guo, Z.L. Ling, O. Cespedes, P. Quirke and D.J. Zhou (see also DNA Research).
Keywords for this news article include: Leeds, Europe, DNA Research, United Kingdom, Nanotechnology, Emerging Technologies, Enzymes and Coenzymes, Magnetic Nanoparticles
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