By a News Reporter-Staff News Editor at Biotech Week -- Current study results on DNA Research have been published. According to news reporting originating from Chongqing, People's Republic of China, by NewsRx correspondents, research stated, "Herein, a novel sensitive pseudobienzyme electrocatalytic DNA biosensor was proposed for mercury ion (Hg2+) detection by using autonomously assembled hemin/G-quadruplex DNAzyme nanowires for signal amplification. Thiol functionalized capture DNA was firstly immobilized on a nano-Au modified glass carbon electrode (GCE)."
Our news editors obtained a quote from the research from Southwest University, "In presence of Hg2+, the specific coordination between Hg2+ and T could result in the assembly of primer DNA on the electrode, which successfully triggered the HCR to form the hemin/Gquadruplex DNAzyme nanowires with substantial redox probe thionine (Thi). In the electrolyte of PBS containing NADH, the hemin/G-quadruplex nanowires firstly acted as an NADH oxidase to assist the concomitant formation of H2O2 in the presence of dissolved O-2. Then, with the redox probe Thi as electron mediator, the hemin/G-quadruplex nanowires acted as an HRP-mimicking DNAzyme that quickly bioelectrocatalyzed the reduction of produced H2O2, which finally led to a dramatically amplified electrochemical signal."
According to the news editors, the research concluded: "This method has demonstrated a high sensitivity of Hg2+ detection with the dynamic concentration range spanning from 1.0 ng L-1 to 10 mg L-1 Hg2+ and a detection limit of 0.5 ng L-1 (2.5 pM) at the 3S(blank) level, and it also demonstrated excellent selectivity against other interferential metal ions."
For more information on this research see: Sensitive pseudobienzyme electrocatalytic DNA biosensor for mercury(II) ion by using the autonomously assembled hemin/G-quadruplex DNAzyme nanowires for signal amplification. Analytica Chimica Acta, 2014;811():23-28. Analytica Chimica Acta can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Analytica Chimica Acta - www.elsevier.com/wps/product/cws_home/502681)
The news editors report that additional information may be obtained by contacting Y.L. Yuan, Southwest University, Coll Resources & Environm, Chongqing 400715, People's Republic of China. Additional authors for this research include M. Gao, G.P. Liu, Y.Q. Chai, S.Q. Wei and R. Yuan (see also DNA Research).
Keywords for this news article include: Asia, Nanowire, Chongqing, Biosensing, DNA Research, Bioengineering, Bionanotechnology, Nanobiotechnology, Emerging Technologies, People's Republic of China
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