By a News Reporter-Staff News Editor at Biotech Week -- Researchers detail new data in Biotechnology. According to news originating from Szeged, Hungary, by NewsRx correspondents, research stated, "Chlamydiae are obligate intracellular bacteria that propagate in the inclusion, a specific niche inside the host cell. The standard method for counting chlamydiae is immunofluorescent staining and manual counting of chlamydial inclusions."
Our news journalists obtained a quote from the research from Biology Research Center, "High- or medium-throughput estimation of the reduction in chlamydial inclusions should be the basis of testing antichlamydial compounds and other drugs that positively or negatively influence chlamydial growth, yet low-throughput manual counting is the common approach. To overcome the time-consuming and subjective manual counting, we developed an automatic inclusion-counting system based on a commercially available DNA chip scanner. Fluorescently labeled inclusions are detected by the scanner, and the image is processed by ChlamyCount, a custom plug-in of the ImageJ software environment. ChlamyCount was able to measure the inclusion counts over a 1-log-unit dynamic range with a high correlation to the theoretical counts. ChlamyCount was capable of accurately determining the MICs of the novel antimicrobial compound PCC00213 and the already known antichlamydial antibiotics moxifloxacin and tetracycline. ChlamyCount was also able to measure the chlamydial growth-altering effect of drugs that influence host-bacterium interaction, such as gamma interferon, DEAE-dextran, and cycloheximide."
According to the news editors, the research concluded: "ChlamyCount is an easily adaptable system for testing antichlamydial antimicrobials and other compounds that influence Chlamydia-host interactions."
For more information on this research see: Application of DNA Chip Scanning Technology for Automatic Detection of Chlamydia trachomatis and Chlamydia pneumoniae Inclusions. Antimicrobial Agents and Chemotherapy, 2014;58(1):405-413. Antimicrobial Agents and Chemotherapy can be contacted at: Amer Soc Microbiology, 1752 N St NW, Washington, DC 20036-2904, USA. (American Society for Microbiology - www.asm.org; Antimicrobial Agents and Chemotherapy - aac.asm.org)
The news correspondents report that additional information may be obtained from A. Bogdanov, Hungarian Academy Sci, Biol Res Center, Cellular Imaging Lab, H-6701 Szeged, Hungary. Additional authors for this research include V. Endresz, S. Urban, I. Lantos, J. Deak, K. Burian, K. Onder, F. Ayaydin, P. Balazs and D.P. Virok (see also Biotechnology).
Keywords for this news article include: Biotechnology, Szeged, Europe, Hungary, DNA Chips, DNA Research, Chlamydiaceae, Chlamydia trachomatis, Gram-Negative Bacteria
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