By a News Reporter-Staff News Editor at Life Science Weekly -- Researchers detail new data in Proteobacteria. According to news reporting originating from Los Alamos, New Mexico, by NewsRx correspondents, research stated, "A microfluidic device was developed to separate heterogeneous particle or cell mixtures in a continuous flow using acoustophoresis. In this device, two identical surface acoustic waves (SAWs) generated by interdigital transducers (IDTs) propagated toward a microchannel, which accordingly built up a standing surface acoustic wave (SSAW) field across the channel."
Our news editors obtained a quote from the research from Los Alamos National Laboratory, "A numerical model, coupling a piezoelectric effect in the solid substrate and acoustic pressure in the fluid, was developed to provide a better understanding of SSAW-based particle manipulation. It was found that the pressure nodes across the channel were individual planes perpendicular to the solid substrate. In the separation experiments, two side sheath flows hydrodynamically focused the injected particle or cell mixtures into a very narrow stream along the centerline. Particles flowing through the SSAW field experienced an acoustic radiation force that highly depends on the particle properties. As a result, dissimilar particles or cells were laterally attracted toward the pressure nodes at different magnitudes, and were eventually switched to different outlets. Two types of fluorescent microspheres with different sizes were successfully separated using the developed device. In addition, Escherichia coli bacteria premixed in peripheral blood mononuclear cells (PBMCs) were also efficiently isolated using the SSAW-base separation technique."
According to the news editors, the research concluded: "Flow cytometric analysis on the collected samples found that the purity of separated E. coli bacteria was 95.65%."
For more information on this research see: Separation of Escherichia coli Bacteria from Peripheral Blood Mononuclear Cells Using Standing Surface Acoustic Waves. Analytical Chemistry, 2013;85(19):9126-9134. Analytical Chemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Analytical Chemistry - www.pubs.acs.org/journal/ancham)
The news editors report that additional information may be obtained by contacting Y. Ai, Los Alamos Natl Lab, Biosci Div, Los Alamos, NM 87545, United States. Additional authors for this research include C.K. Sanders and B.L. Marrone (see also Proteobacteria).
Keywords for this news article include: Los Alamos, New Mexico, United States, Proteobacteria, Escherichia coli, Enterobacteriaceae, Gram-Negative Bacteria, North and Central America
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