By a News Reporter-Staff News Editor at Biotech Week -- New research on Biomedicine and Biomedical Engineering is the subject of a report. According to news reporting originating from Nashville, Tennessee, by NewsRx correspondents, research stated, "Basement membrane, a specialized ECM that underlies polarized epithelium of eumetazoans, provides signaling cues that regulate cell behavior and function in tissue genesis and homeostasis. A collagen IV scaffold, a major component, is essential for tissues and dysfunctional in several diseases."
Our news editors obtained a quote from the research from Tennessee State University, "Studies of bovine and Drosophila tissues reveal that the scaffold is stabilized by sulfilimine chemical bonds (S = N) that covalently cross-link methionine and hydroxylysine residues at the interface of adjoining triple helical protomers. Peroxidasin, a heme peroxidase embedded in the basement membrane, produces hypohalous acid intermediates that oxidize methionine, forming the sulfilimine cross-link. We explored whether the sulfilimine cross-link is a fundamental requirement in the genesis and evolution of epithelial tissues by determining its occurrence and evolutionary origin in Eumetazoa and its essentiality in zebrafish development; 31 species, spanning 11 major phyla, were investigated for the occurrence of the sulfilimine cross-link by electrophoresis, MS, and multiple sequence alignment of de novo transcriptome and available genomic data for collagen IV and peroxidasin. The results show that the cross-link is conserved throughout Eumetazoa and arose at the divergence of Porifera and Cnidaria over 500 Mya. Also, peroxidasin, the enzyme that forms the bond, is evolutionarily conserved throughout Metazoa. Morpholino knockdown of peroxidasin in zebrafish revealed that the cross-link is essential for organogenesis."
According to the news editors, the research concluded: "Collectively, our findings establish that the triad-a collagen IV scaffold with sulfilimine cross-links, peroxidasin, and hypohalous acids-is a primordial innovation of the ECM essential for organogenesis and tissue evolution."
For more information on this research see: A unique covalent bond in basement membrane is a primordial innovation for tissue evolution. Proceedings of the National Academy of Sciences of the United States of America, 2014;111(1):331-336. Proceedings of the National Academy of Sciences of the United States of America can be contacted at: Natl Acad Sciences, 2101 Constitution Ave NW, Washington, DC 20418, USA. (National Academy of Sciences - www.nasonline.org/; Proceedings of the National Academy of Sciences of the United States of America - www.nasonline.org/publications/pnas/)
The news editors report that additional information may be obtained by contacting A.L. Fidler, Tennessee State Univ, Dept. of Biol Sci, Nashville, TN 37209, United States. Additional authors for this research include R.M. Vanacore, S.V. Chetyrkin, V.K. Pedchenko, G. Bhave, V.P. Yin, C.L. Stothers, K.L. Rose, W.H. McDonald, T.A. Clark, D.B. Borza, R.E. Steele, M.T. Ivy, J.K. Hudson and B.G. Hudson (see also Biomedicine and Biomedical Engineering).
Keywords for this news article include: Tissue Engineering, Biomedicine and Biomedical Engineering, Collagen, Nashville, Tennessee, United States, Bioengineering, North and Central America, Extracellular Matrix Proteins
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