By a News Reporter-Staff News Editor at Life Science Weekly -- A new study on Silicon is now available. According to news reporting out of Knoxville, Tennessee, by NewsRx editors, research stated, "The importance of fluorescent detection in many fields is well established. While advancements in instrumentation and the development of brighter fluorophore have increased sensitivity and lowered the detection limits of the method, additional gains can be made by manipulating the local electromagnetic field."
Our news journalists obtained a quote from the research from the University of Tennessee, "Herein we take advantage of silicon nanopillars that exhibit optical resonances and field enhancement on their surfaces and demonstrate their potential in improving performance of biomolecular fluorescent assays. We use electron beam lithography and wafer scale processes to create silicon nanoscale pillars with dimensions that can be tuned to maximize fluorescence enhancement in a particular spectral region. Performance of the nanopillar based fluorescent assay was quantified using two model bioaffinity systems (biotinstreptavidin and immunoglobulin G-antibody) as well as covalent binding of fluorescently tagged bovine serum albumin (BSA). The effects of pillar geometry and number of pillars in arrays were evaluated. Color specific and pillar diameter dependent enhancement of fluorescent signals is clearly demonstrated using green and red labels (FITC, DyLight 488, Alexa 568, and Alexa 596). The ratios of the on pillar to off pillar signals normalized by the nominal increase in surface area due to nanopillars were found to be 43, 75, and 292 for the IgG-antibody assay, streptavidin-biotin system, and covalently attached BSA, respectively. Applicability of the presented approaches to the detection of small numbers of molecules was evaluated using highly diluted labeled proteins and also control experiments without biospecific analytes."
According to the news editors, the research concluded: "Our analysis indicates that detection of fewer than 10 tagged proteins is possible."
For more information on this research see: Silicon Nanopillars As a Platform for Enhanced Fluorescence Analysis. Analytical Chemistry, 2013;85(19):9031-9038. Analytical Chemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Analytical Chemistry - www.pubs.acs.org/journal/ancham)
Our news journalists report that additional information may be obtained by contacting M. Kandziolka, University of Tennessee, Dept. of Chem, Knoxville, TN 37996, United States. Additional authors for this research include J.J. Charlton, I.I. Kravchenko, J.A. Bradshaw, I.A. Merkulov, M.J. Sepaniak and N.V. Lavrik (see also Silicon).
Keywords for this news article include: Antibodies, Silicon, Knoxville, Tennessee, Immunology, United States, Blood Proteins, Immunoglobulins, North and Central America
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