By a News Reporter-Staff News Editor at Life Science Weekly -- Researchers detail new data in Life Science Research. According to news reporting from Blacksburg, Virginia, by NewsRx journalists, research stated, "Neuronal Wiskott-Aldrich syndrome protein (N-WASP)-activated actin polymerization drives extension of invadopodia and podosomes into the basement layer. In addition to activating Arp2/3, N-WASP binds actin-filament barbed ends, and both N-WASP and barbed ends are tightly clustered in these invasive structures."
The news correspondents obtained a quote from the research from Virginia Polytechnic Institute and State University, "We use nanofibers coated with N-WASP WWCA domains as model cell surfaces and single-actin-filament imaging to determine how clustered N-WASP affects Arp2/3-independent barbed-end assembly. Individual barbed ends captured by WWCA domains grow at or below their diffusion-limited assembly rate. At high filament densities, however, overlapping filaments form buckles between their nanofiber tethers and myosin attachment points. These buckles grew similar to 3.4-fold faster than the diffusion-limited rate of unattached barbed ends. N-WASP constructs with and without the native polyproline (PP) region show similar rate enhancements in the absence of profilin, but profilin slows barbed-end acceleration from constructs containing the PP region. Increasing Mg2+ to enhance filament bundling increases the frequency of filament buckle formation, consistent with a requirement of accelerated assembly on barbed-end bundling."
According to the news reporters, the research concluded: "We propose that this novel N-WASP assembly activity provides an Arp2/3-independent force that drives nascent filament bundles into the basement layer during cell invasion."
For more information on this research see: Processive acceleration of actin barbed-end assembly by N-WASP. Molecular Biology of the Cell, 2014;25(1):55-65. Molecular Biology of the Cell can be contacted at: Amer Soc Cell Biology, 8120 Woodmont Ave, Ste 750, Bethesda, MD 20814-2755, USA (see also Life Science Research).
Our news journalists report that additional information may be obtained by contacting N. Khanduja, Virginia Polytechnic Institute & State Univ, Dept. of Biol Sci, Blacksburg, VA 24061, United States.
Keywords for this news article include: Virginia, Blacksburg, United States, Life Science Research, North and Central America
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