By a News Reporter-Staff News Editor at Biotech Week -- Current study results on Enzymes and Coenzymes have been published. According to news reporting out of San Antonio, Texas, by NewsRx editors, research stated, "The signal transduction and enzyme activity were investigated in biosensors based on the glucose oxidase (GOx) and carbon nanotubes (CNT) embedded in a bioadhesive film of chitosan (CHIT). The voltammetric studies showed that, regardless of CHIT matrix, the GOx adsorbed on CNT yielding a pair of surface-confined current peaks at -0.48 V. The anodic peak did not increase in the presence of glucose in an O-2-free solution indicating the lack of direct electron transfer (DET) between the enzymatically active GOx and CNT."
Our news journalists obtained a quote from the research from the University of Texas, "The voltammetric peaks were due to the redox of enzyme cofactor flavin adenine dinucleotide (FAD), which was not the part of active enzyme. The presented data suggest that DET may not be happening for any type of GOx/CNT-based sensor. The biosensor was sensitive to glucose in air-equilibrated solutions indicating the O-2-mediated enzymatic oxidation of glucose.. The signal transduction relied on the net drop in a biosensor current that was caused by a decrease in a 4-e(-) O-2 reduction current and an increase in a 2-e(-) H2O2 reduction current. The enzyme assays showed that CNT nearly doubled the retention of GOx in a biosensor while decreasing the average enzymatic activity of retained enzyme by a factor of 4-5. Such inhibition should be considered when using a protein-assisted solubilization of CNT in water for biomedical applications."
According to the news editors, the research concluded: "The proposed analytical protocols can be also applied to study the effects of nanoparticles on proteins in assessing the health risks associated with the use of nanomaterials."
For more information on this research see: On the Direct Electron Transfer, Sensing, and Enzyme Activity in the Glucose Oxidase/Carbon Nanotubes System. Analytical Chemistry, 2014;86(1):752-757. Analytical Chemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Analytical Chemistry - www.pubs.acs.org/journal/ancham)
Our news journalists report that additional information may be obtained by contacting M. Wooten, Univ Texas San Antonio, Dept. of Chem, San Antonio, TX 78249, United States. Additional authors for this research include S. Karra, M.G. Zhang and W. Gorski (see also Enzymes and Coenzymes).
Keywords for this news article include: Texas, Biosensing, Fullerenes, San Antonio, United States, Bioengineering, Glucose Oxidase, Carbon Nanotubes, Bionanotechnology, Nanobiotechnology, Emerging Technologies, Enzymes and Coenzymes, Alcohol Oxidoreductases, North and Central America
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