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New Findings from Nanjing Medical University in Gene Therapy Provides New Insights

February 20, 2014

By a News Reporter-Staff News Editor at Gene Therapy Weekly -- Fresh data on Biotechnology are presented in a new report. According to news reporting out of Jiangsu, People's Republic of China, by NewsRx editors, research stated, "To enhance serum-resistance and overcome the lysosomal barrier are effective and feasible strategies to increase the transfection efficiency of non-viral gene delivery system. For the systemic delivery of therapeutic gene, we previously developed self-assemble carboxymethyl poly(L-histidine) (CMPLH)/poly(beta-amino ester) (PbAE)/pDNA ternary complex nanoparticles based on electrostatic coating as an effective pDNA carrier."

Our news journalists obtained a quote from the research from Nanjing Medical University, "Recharging cationic PbAE/pDNA polyplexes with CM-PLH was a promising method to reduce the cytotoxicity and enhance the stability in vivo of positive charged polyplexes. In the present study, the transfection activities of ternary complex nanoparticles were further evaluated in vitro and in vivo. The transfection efficiency of ternary complex nanoparticles showed significant serumresistance (CM-PLH-containing (51.9 +/- 4.35)% in 50% FBS > CM-PLH-free (14.7 +/- 5.66)% in 50% FBS), cell line dependent (HEK293 > MCF-7 > COS7 > Bl6F10 > A549 > Hela > SPC-A1 > CHO > SKOV3) and incubation period dependent (24 h, 20 h, 16 h> 12 h>8h >4h >2h >lh > 0.5 h). After transfected with ternary complex nanoparticles loading pGV240-MDA-71IL-24, the B1 6F10 cells exhibited significant apoptosis and proliferation inhibition due to the expression of IL-24. Moreover, in the pulmonary metastatic melanoma model, ternary complex nanoparticles loading pGV240-MDA-71IL-24 showed significant antitumor therapeutic efficacy in vivo."

According to the news editors, the research concluded: "These results suggested that CM-PLH/PbAE/pDNA ternary complex nanoparticles were promising and challenging gene vector for practical application."

For more information on this research see: Serum-resistant complex nanoparticles functionalized with imidazole-rich polypeptide for gene delivery to pulmonary metastatic melanoma. International Journal of Pharmaceutics, 2014;461(1-2):559-569. International Journal of Pharmaceutics can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier -; International Journal of Pharmaceutics -

Our news journalists report that additional information may be obtained by contacting J.J. Gu, Nanjing Medical University, Sch Pharm, Dept. of Pharmaceut, Nanjing 210029, Jiangsu, People's Republic of China. Additional authors for this research include X.Y. Chen, H.L. Xin, X.L. Fang and X.Y. Sha (see also Biotechnology).

Keywords for this news article include: Asia, Biotechnology, Jiangsu, Healthcare, Gene Therapy, Nanoparticle, Bioengineering, Nanotechnology, Metastatic Melanoma, Emerging Technologies, Diseases and Conditions, People's Republic of China

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Source: Gene Therapy Weekly

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