By a News Reporter-Staff News Editor at Cancer Gene Therapy Week -- Researchers detail new data in Biotechnology. According to news reporting from Changchun, People's Republic of China, by NewsRx journalists, research stated, "Prostate cancer (PCa) is the second most lethal malignancy in men. It has been reported that chemokines, produced by cancer cells, have linked with tumor progression and metastatic spread."
The news correspondents obtained a quote from the research from Jilin University, "We have proven that chemokine (C-C) motif ligand 2 (CCL2) is involved in the growth and invasion of PCa. In this study, we studied whether CC chemokine receptor 2 (CCR2), the receptor of CCL2, also contributes to PCa progression. We constructed the recombinant plasmid pGCsi-CCR2 and investigated the effects of pGCsi-CCR2 on proliferation, apoptosis, migration, and invasion of PC-3M cells. RT-PCR and Western blot assay showed that transfection with the plasmid pGCsi-CCR2 successfully inhibited the CCR2 expression. The cell proliferation rate was significantly slow, and the apoptotic rate was increased in PC-3M cells treated with CCR2-siRNA, indicated by MTT cell viability and TUNEL assay, respectively. As expected, CCR2 knockdown also reduced the migration and invasion of PC-3M cells, as illustrated through wound-healing assay and Transwell assay. The possible molecular mechanism was the regulation of several signal pathways involved in survival, apoptosis, migration, and metastasis."
According to the news reporters, the research concluded: "Altogether, the present finding suggests that CCR2 expression is crucial for CCL2-induced proliferation and invasion of PC-3M cells, and CCR2 could also be a promising molecular target for prevention of PCa growth and metastasis."
For more information on this research see: RNAi Targeting of CCR2 Gene Expression Induces Apoptosis and Inhibits the Proliferation, Migration, and Invasion of PC-3M Cells. Oncology Research, 2014;21(2):73-82. Oncology Research can be contacted at: Cognizant Communication Corp, 18 Peekskill Hollow Rd, PO Box 37, Putnam Valley, NY 10579, USA (see also Biotechnology).
Our news journalists report that additional information may be obtained by contacting J. Gao, Jilin University, Hosp 4, Dept. of Urol, Changchun, Jilin, People's Republic of China. Additional authors for this research include A. Wang, M.C. Zhang, H.Y. Li, K.C. Wang, Y.P. Han, Z.X. Wang, C.L. Shi and W.H. Wang.
Keywords for this news article include: Asia, Biotechnology, Changchun, Apoptosis, Immunology, Membrane Proteins, Cytokine Receptors, Cancer Gene Therapy, Chemokine Receptors, People's Republic of China
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