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Findings from Vanderbilt University in Membrane Proteins Reported

February 18, 2014



By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Membrane Proteins. According to news originating from Nashville, Tennessee, by NewsRx correspondents, research stated, "Pulsed EPR DEER structural studies of membrane proteins in a lipid bilayer have often been hindered by difficulties in extracting accurate distances when compared to those of globular proteins. In this study, we employed a combination of three recently developed methodologies, (1) bifunctional spin labels (BSL), (2) SMA-Lipodisq nanoparticles, and (3) Q band pulsed EPR measurements, to obtain improved signal sensitivity, increased transverse relaxation time, and more accurate and precise distances in DEER measurements on the integral membrane protein KCNE1."

Our news journalists obtained a quote from the research from Vanderbilt University, "The KCNE1 EPR data indicated an similar to 2-fold increase in the transverse relaxation time for the SMA-Lipoclisq nanoparticles when compared to those of proteoliposomes and narrower distance distributions for the BSL when compared to those of the standard MTSL. The certainty of information content in DEER data obtained for KCNE1 in SMA-Lipodisq nanoparticles is comparable to that in micelles."

According to the news editors, the research concluded: "The combination of techniques will enable researchers to potentially obtain more precise distances in cases where the traditional spin labels and membrane systems yield imprecise distance distributions."

For more information on this research see: DEER EPR Measurements for Membrane Protein Structures via Bifunctional Spin Labels and Lipodisq Nanoparticles. Biochemistry, 2013;52(38):6627-6632. Biochemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Biochemistry - www.pubs.acs.org/journal/bichaw)

The news correspondents report that additional information may be obtained from I.D. Sahu, Vanderbilt University, Struct Biol Center, Nashville, TN 37232, United States. Additional authors for this research include R.M. McCarrick, K.R. Troxel, R.F. Zhang, H.J. Smith, M.M. Dunagan, M.S. Swartz, P.V. Rajan, B.M. Kroncke, C.R. Sanders and G.A. Lorigan (see also Membrane Proteins).

Keywords for this news article include: Nashville, Tennessee, Nanoparticle, United States, Nanotechnology, Membrane Proteins, Emerging Technologies, North and Central America

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Source: Life Science Weekly


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