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Findings from University of Sherbrooke Provides New Data on Peptides and Proteins

February 18, 2014



By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Peptides and Proteins have been published. According to news reporting originating from Sherbrooke, Canada, by NewsRx correspondents, research stated, "MALDI mass spectrometry imaging (MALDI-MSI) was presented as a good strategy to highlight regions presenting specific phenotypes based on molecular content. The proteins present in the different areas can be identified by MALDI MSI; however, the number of protein identifications remains low in comparison with classical MS-based proteomics approaches."

Our news editors obtained a quote from the research from the University of Sherbrooke, "To overcome this, a new strategy, involving the microdissection of tissue sections mounted on parafilm M-covered glass slides, is presented. Extraction and fractionation of proteins from a specific region of interest were investigated, leading to the identification of more than 1000 proteins from each microdissected piece. The strength of this cheap technique lies in the facile excision of millimeter-sized portions from the tissue allowing for the identification of proteins from cells of a specific phenotype obtained from the MALDI MS imaging-based molecular classification using hierarchical clustering. This approach can be. extended to whole tissue sections in order to generate images of the section based on label-free quantification obtained from identification data. As a proof of concept, we have studied a tissue mounted on a parafilm M-covered glass slide, cut it into regular pieces, and submitted each piece to identification and quantification according to the developed parafilm-assisted microdissection (PAM) method. Images were then reconstructed by relative quantification of identified proteins based on spectral counting of the peptides analyzed by nanoLC-MS and MS/MS."

According to the news editors, the research concluded: "This strategy of quantification-based MSI offers new possibilities for mapping a large number of high and low abundance proteins."

For more information on this research see: Quantification-Based Mass Spectrometry Imaging of Proteins by Parafilm Assisted Microdissection. Analytical Chemistry, 2013;85(17):8127-8134. Analytical Chemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Analytical Chemistry - www.pubs.acs.org/journal/ancham)

The news editors report that additional information may be obtained by contacting J. Franck, University of Sherbrooke, Fac Med & Sci Sante, Dept. of Chirurg, Inst Pharmacol SherbrookeServ Urol, Sherbrooke, PQ J1H 5N4, Canada. Additional authors for this research include J. Quanico, M. Wisztorski, R. Day, M. Salzet and I. Fournier (see also Peptides and Proteins).

Keywords for this news article include: Quebec, Canada, Sherbrooke, Amino Acids, Peptides and Proteins, North and Central America

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Source: Life Science Weekly


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