By a News Reporter-Staff News Editor at Life Science Weekly -- Data detailed on Gram-Positive Bacteria have been presented. According to news originating from Wuhan, People's Republic of China, by NewsRx correspondents, research stated, "Identification of the resistance mechanism of insects against Bacillus thuringiensis Cry1A toxin is becoming an increasingly challenging task. This fact highlights the need for establishing new methods to further explore the molecular interactions of Cry1A toxin with insects and the receptor-binding region of Cry1A toxins for their wider application as biopesticides and a gene source for gene-modified crops."
Our news journalists obtained a quote from the research from Huazhong Agricultural University, "In this contribution, a quantum dot-based near-infrared fluorescence imaging method has been applied for direct dynamic tracking of the specific binding of Cry1A toxins, CrylAa and CrylAc, to the midgut tissue of silkworm. The in vitro fluorescence imaging displayed the higher binding specificity of CrylAa-QD probes compared to CrylAc-QD to the brush border membrane vesicles of midgut from silkworm. The in vivo imaging demonstrated that more CrylAa-QDs binding to silkworm midgut could be effectively and distinctly monitored in living silkworms. Furthermore, frozen section analysis clearly indicated the broader receptor-binding region of CrylAa compared to that of CrylAc in the midgut part."
According to the news editors, the research concluded: "These observations suggest that the insecticidal activity of Cry toxins may depend on the receptor-binding sites, and this scatheless and visual near-infrared fluorescence imaging could provide a new avenue to study the resistance mechanism to maintain the insecticidal activity of B. thuringiensis toxins."
For more information on this research see: Intravital imaging of Bacillus thuringiensis Cry1A toxin binding sites in the midgut of silkworm. Analytical Biochemistry, 2014;447():90-97. Analytical Biochemistry can be contacted at: Academic Press Inc Elsevier Science, 525 B St, Ste 1900, San Diego, CA 92101-4495, USA. (Elsevier - www.elsevier.com; Analytical Biochemistry - www.elsevier.com/wps/product/cws_home/622781)
The news correspondents report that additional information may be obtained from N. Li, Huazhong Agricultural University, Coll Food Sci & Technol, Wuhan 430070, People's Republic of China. Additional authors for this research include J. Wang, H.Y. Han, L. Huang, F. Shao and X.P. Li (see also Gram-Positive Bacteria).
Keywords for this news article include: Asia, Wuhan, Bacillaceae, Gram-Positive Rods, Bacillus thuringiensis, Gram-Positive Bacteria, People's Republic of China, Endospore-Forming Bacteria, Gram-Positive Endospore-Forming Rods
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