By a News Reporter-Staff News Editor at Stem Cell Week -- Investigators discuss new findings in Stem Cell Research. According to news reporting originating from Copenhagen, Denmark, by NewsRx correspondents, research stated, "Post-translational modifications (PTMs) of histones play a major role in regulating chromatin dynamics and influence processes such as transcription and DNA replication. Here, we report 114 distinct combinations of coexisting PTMs of histone H3 obtained from mouse embryonic stem (ES) cells."
Our news editors obtained a quote from the research from the University of Copenhagen, "Histone H3 N-terminal tail peptides (amino acids 1-50, 5-6 kDa) were separated by optimized weak cation exchange/hydrophilic interaction liquid chromatography (WCX/HILIC) and sequenced online by electron transfer dissociation (ETD) tandem mass spectrom etry (MS/MS). High mass accuracy and near complete sequence coverage allowed unambiguous mapping of the major histone marks and discrimination between isobaric and nearly isobaric PTMs such as trimethylation and acetylation. Hierarchical data analysis identified H3K27me2-H3K36me2 as the most frequently observed PTMs in H3. Modifications at H3 residues K27 and K36 often coexist with the abundant mark K23ac, and we identified two frequently occurring quadruplet marks 'K9me1K23acK27me2K36me2' and 'K9me3K23acK27me2K36me', which might indicate a role in crosstalk. Co-occurrence frequency analysis revealed also an interplay between methylations of K9, K27, and K36, suggesting interdependence between histone methylation marks."
According to the news editors, the research concluded: "We hypothesize that the most abundant coexisting PTMs may provide a signature for the permissive state of mouse ES cells."
For more information on this research see: Precision Mapping of Coexisting Modifications in Histone H3 Tails from Embryonic Stem Cells by ETD-MS/MS. Analytical Chemistry, 2013;85(17):8232-8239. Analytical Chemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Analytical Chemistry - www.pubs.acs.org/journal/ancham)
The news editors report that additional information may be obtained by contacting H.R. Jung, University of Copenhagen, Danish Stem Cell Center, DK-2200 Copenhagen N, Denmark. Additional authors for this research include S. Sidoli, S. Haldbo, R.R. Sprenger, V. Schwammle, D. Pasini, K. Helin and O.N. Jensen (see also Stem Cell Research).
Keywords for this news article include: Europe, Denmark, Histones, Copenhagen, Nucleoproteins, Stem Cell Research, Embryonic Stem Cells
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