News Column

Data on Apoptosis Detailed by Researchers at Dankook University

February 19, 2014



By a News Reporter-Staff News Editor at Biotech Week -- Current study results on Apoptosis have been published. According to news reporting originating in Chungnam, South Korea, by NewsRx journalists, research stated, "The 9-hydroxypheophorbide-alpha (9-HPbD) is a chlorophyll derivative and was found to be very effective for photodynamic therapy of tumor cells. The current study investigates uptake, retention, and intracellular localization of 9-HPbD by HeLa, human cervical cancer cells via fluorescence spectrophotometry and confocal laser scanning microscopy, and its photodynamic effect against human cervical carcinoma cell."

The news reporters obtained a quote from the research from Dankook University, "HeLa cells exposed to 9-HPbD exhibited a linear uptake of photosensitizer during the first 12 h, and after removal of 9-HPbD, cell fluorescence was observed to decrease gradually over the next 12 h. Cells treated with 9-HPbD and stained with a panel of organelle-specific fluorescence probes (MitoTracker, LysoTracker, and ER-Tracker) revealed an intracellular fluorescence distribution restricted to cytoplasmic compartments with no detectable fluorescence in the nucleus. The 9-HPbD showed cytotoxicity effect against HeLa cells in 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay. Endoplasmic reticulum (ER) disruption and cellular calcium dynamics also showed a photoactivation followed by cell death. The apoptotic effect of 9-HPbD was confirmed by caspase 3 activity study and immunofluorescence study of caspase 12. Morphological observation through the transmission electron microscopy and scanning electron microscopy also confirmed that 9-HPbD can induce apoptosis in HeLa cells."

According to the news reporters, the research concluded: "Therefore, it can be concluded that maximum uptake and clearance time of 9-HPbD was 12 h with endoplasmic reticulum as the major organelle site in cellular uptake, and 9-HPbD can induce apoptosis in HeLa cells through ER stress-related pathways via activation of caspase 12."

For more information on this research see: Cellular uptake of 9-hydroxypheophorbide-alpha and its photoactivation to induce ER stress-related apoptosis in human cervical cancer cells. Lasers in Medical Science, 2014;29(1):289-299. Lasers in Medical Science can be contacted at: Springer London Ltd, 236 Grays Inn Rd, 6TH Floor, London WC1X 8HL, England. (Springer - www.springer.com; Lasers in Medical Science - www.springerlink.com/content/0268-8921/)

Our news correspondents report that additional information may be obtained by contacting J.C. Ahn, Dankook University, Coll Med, Dept. of Otolaryngol Head & Neck Surg, Cheonan 330714, Chungnam, South Korea. Additional authors for this research include R. Biswas, J.H. Moon and P.S. Chung (see also Apoptosis).

Keywords for this news article include: Asia, Cancer, Chungnam, Caspases, Oncology, Apoptosis, Hela Cells, South Korea, Tumor Cell Line, Peptide Hydrolases, Enzymes and Coenzymes, Cysteine Endopeptidases

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Source: Biotech Week


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