By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Neuroglia. According to news originating from Minneapolis, Minnesota, by NewsRx correspondents, research stated, "We have developed a novel in vitro approach for monitoring fast neurochemical dynamics in model cell systems using microdialysis sampling coupled with high-speed capillary electrophoresis (CE). Cells from an immortalized astrocyte line (C8-D1A) were cultured in direct contact with the porous membrane of a microdialysis probe."
Our news journalists obtained a quote from the research from the University of Minnesota, "Confocal microscopy was used to confirm cell viability and confluency over the microdialysis sampling region. Small molecules released from the astrocytes were efficiently sampled by the probe due to the direct contact with the membrane. Microdialysis sampling was coupled with online, high-speed CE allowing changes in the dialysate concentration of small molecule amine neurochemicals to be monitored with 20 s temporal resolution. Basal release of a number of important analytes was detected including glycine, taurine, D-serine, and glutamate. The ability of the in vitro microdialysis-CE instrument to monitor dynamic changes in analyte concentration was assessed by transferring a probe cultured with astrocytes from a solution containing artificial cerebrospinal fluid (aCSF) to a high K+ solution (100 mM K+-aCSF). Upon stimulation, the observed concentration of a number of key neurochemicals increased dramatically including glycine (700%), taurine (185%), and serine (215%)."
According to the news editors, the research concluded: "Amino acids such as phenylalanine and valine, which are not known to respond to cellular swelling mechanisms, were unaffected by the K+ stimulation."
For more information on this research see: Monitoring Neurochemical Release from Astrocytes Using in Vitro Microdialysis Coupled with High-Speed Capillary Electrophoresis. Analytical Chemistry, 2013;85(19):9070-9077. Analytical Chemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Analytical Chemistry - www.pubs.acs.org/journal/ancham)
The news correspondents report that additional information may be obtained from A.L. Hogerton, University of Minnesota, Dept. of Chem, Minneapolis, MN 55455, United States (see also Neuroglia).
Keywords for this news article include: Minnesota, Neuroglia, Astrocytes, Minneapolis, United States, North and Central America, Capillary Electrophoresis
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