By a News Reporter-Staff News Editor at Science Letter -- Research findings on Enzymes and Coenzymes are discussed in a new report. According to news originating from Jiangsu, People's Republic of China, by NewsRx correspondents, research stated, "Immobilization of trypsin onto the superparamagnetic carboxymethyl chitosan (Fe3O4 (PEG + CM-CTS)) nanoparticles was studied. FTIR and fluorescence spectroscopy data demonstrated that the Fe3O4 (PEG + CM-CTS) nanoparticles were capable of preventing the trypsin unfolding."
Our news journalists obtained a quote from the research from Jiangnan University, "Due to the large specific surface area and excellent dispersibility, the adsorption equilibrium of trypsin onto the nanoparticles was achieved quickly within 30 min. The results of kinetic parameters (Michaelis constant, Km) with regards to the free trypsin (FT) and immobilized trypsin (IT) were 23.1 and 24.1 mg/mL separately, implying that IT has less affinity to the BAEE used as the substrate. However, the MALDI-TOF MS analysis indicated that, IT could be used for fast and efficient Bovine Serum Albumin (BSA) digestion under very facile processes, thanks to the easy manipulation of the magnetic nanoparticles (MNs), as well as the greatly reduced digestion time (from 12 h to 15 min). IT exhibited a sound stability after re-uses for six times, with 76.3% of the initial activity thereof still retained, thus making it more attractive in the application fields."
According to the news editors, the research concluded: "These results are expected to open up a great potential use of such Fe3O4 (PEG + CM-CTS) nanoparticles as a superior nanosupport for trypsin immobilization."
For more information on this research see: Novel superparamagnetic sanoparticles for trypsin immobilization and the application for efficient proteolysis. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2013;942():9-14. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands (see also Enzymes and Coenzymes).
The news correspondents report that additional information may be obtained from J. Sun, Jiangnan Univ, Sch Food Sci, Wuxi 214122, Jiangsu, People's Republic of China. Additional authors for this research include K.Y. Hu, Y.T. Liu, Y.T. Pan and Y.J. Yang.
Keywords for this news article include: Asia, Jiangsu, Trypsin, Nanoparticle, Nanotechnology, Peptide Hydrolases, Emerging Technologies, Enzymes and Coenzymes, Serine Endopeptidases, People's Republic of China
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