By a News Reporter-Staff News Editor at Science Letter -- A new study on Biological Factors is now available. According to news reporting originating from Wuhan, People's Republic of China, by NewsRx correspondents, research stated, "A high-throughput method for the determination of 28 mycotoxins involving pressurised liquid extraction (PLE) coupled with liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) has been optimised and validated for determination in various biological fluids and tissues of human and laboratory animals. High-throughput analysis was achieved using PLE pre-treatment and without the need for any cleanup."
Our news editors obtained a quote from the research from Wuhan University, "The extraction solvent was acetonitrile/water/acetic acid (80/19/1, v/v/v). The static extraction time was 5 min. The extraction pressure and temperature were 1500 psi and 140 degrees C, respectively. The flush volume was 60%. The limits of detection, which were defined as CC alpha, varied from 0.01 mu g/kg (mu g/L) to 0.69 mu g/kg (mu g/L). The recoveries of spiked samples from 0.20 mu g/kg (mu g/L) to 2 mu g/kg (mu g/L) ranged from 71% to 100.5% with relative standard deviations of less than 17.5%, except FB1 and FB2 recoveries, which were lower than 60%. The method was successfully applied in real samples, and the data indicate that this technique is a useful analytical method for the determination of mycotoxins from humans and animals."
According to the news editors, the research concluded: "To the best of our knowledge, this method is the first for the large-scale testing of multi-class mycotoxins in all types of biological fluids and tissues that uses PLE and HPLC-MS/MS."
For more information on this research see: A high-throughput method for the simultaneous determination of multiple mycotoxins in human and laboratory animal biological fluids and tissues by PLE and HPLC-MS/MS. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2013;942():113-125. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands (see also Biological Factors).
The news editors report that additional information may be obtained by contacting X.Q. Cao, Wuhan University, Dept. of Pharm, Wuhan 430072, People's Republic of China. Additional authors for this research include S.C. Wu, Y. Yue, S. Wang, Y.T. Wang, L. Tao, H. Tian, J.M. Xie and H. Ding.
Keywords for this news article include: Asia, Wuhan, Mycotoxins, Biological Toxins, Biological Factors, People's Republic of China
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