By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Life Science Research. According to news reporting out of Yichang, People's Republic of China, by NewsRx editors, research stated, "A narrow leaf mutant was isolated from transgenic rice (Oryza sativa L.) lines carrying a T-DNA insertion. The mutant is characterized by narrow leaves during its whole growth period, and was named nal9 (narrow leaf 9)."
Our news journalists obtained a quote from the research from China Three Gorges University, "The mutant also has other phenotypes, such as light green leaves at the seedling stage, reduced plant height, a small panicle and increased tillering. Genetic analysis revealed that the mutation is controlled by a single recessive gene. A hygromycin resistance assay showed that the mutation was not caused by T-DNA insertion, so a map-based cloning strategy was employed to isolate the nal9 gene. The mutant individuals from the F-2 generations of a cross between the nal9 mutant and Longtepu were used for mapping. With 24 F-2 mutants, the nal9 gene was preliminarily mapped near the marker RM156 on the chromosome 3. New INDEL markers were then designed based on the sequence differences between japonica and indica at the region near RM156. The nal9 gene was finally located in a 69.3kb region between the markers V239B and V239G within BAC OJ1212_C05 by chromosome walking. Sequence and expression analysis showed that an ATP-dependent Clp protease proteolytic subunit gene (ClpP) was most likely to be the nal9 gene. Furthermore, the nal9 mutation was rescued by transformation of the ClpP cDNA driven by the 35S promoter. Accordingly, the ClpP gene was identified as the NAL9 gene."
According to the news editors, the research concluded: "Our results provide a basis for functional studies of NAL9 in future work."
For more information on this research see: Characterization and Fine Mapping of a Novel Rice Narrow Leaf Mutant nal9. Journal of Integrative Plant Biology, 2013;55(11):1016-1025. Journal of Integrative Plant Biology can be contacted at: Wiley-Blackwell, 111 River St, Hoboken 07030-5774, NJ, USA. (Wiley-Blackwell - www.wiley.com/; Journal of Integrative Plant Biology - onlinelibrary.wiley.com/journal/10.1111/(ISSN)1744-7909)
Our news journalists report that additional information may be obtained by contacting W. Li, China Three Gorges Univ, Biotechnol Res Center, Yichang 443002, People's Republic of China. Additional authors for this research include C. Wu, G.C. Hu, L. Xing, W.J. Qian, H.M. Si, Z.X. Sun, X.C. Wang, Y.P. Fu and W.Z. Liu (see also Life Science Research).
Keywords for this news article include: Asia, Yichang, Life Science Research, People's Republic of China
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