By a News Reporter-Staff News Editor at Information Technology Newsweekly -- Research findings on Bioinformatics are discussed in a new report. According to news reporting out of Uppsala, Sweden, by VerticalNews editors, research stated, "Finding peaks in ChIP-seq is an important process in biological inference. In some cases, such as positioning nucleosomes with specific histone modifications or finding transcription factor binding specificities, the precision of the detected peak plays a significant role."
Our news journalists obtained a quote from the research from Uppsala University, "There are several applications for finding peaks (called peak finders) based on different algorithms (e.g. MACS, Erange and HPeak). Benchmark studies have shown that the existing peak finders identify different peaks for the same dataset and it is not known which one is the most accurate. We present the first meta-server called Peak Finder MetaServer (PFMS) that collects results from several peak finders and produces consensus peaks. Our application accepts three standard ChIP-seq data formats: BED, BAM, and SAM. Sensitivity and specificity of seven widely used peak finders were examined. For the experiments we used three previously studied Transcription Factors (TF) ChIP-seq datasets and identified three of the selected peak finders that returned results with high specificity and very good sensitivity compared to the remaining four. We also ran PFMS using the three selected peak finders on the same TF datasets and achieved higher specificity and sensitivity than the peak finders individually. We show that combining outputs from up to seven peak finders yields better results than individual peak finders. In addition, three of the seven peak finders outperform the remaining four, and running PFMS with these three returns even more accurate results."
According to the news editors, the research concluded: "Another added value of PFMS is a separate report of the peaks returned by each of the included peak finders."
For more information on this research see: Peak Finder Metaserver - a novel application for finding peaks in ChIP-seq data. Bmc Bioinformatics, 2013;14():280. (BioMed Central - www.biomedcentral.com/; Bmc Bioinformatics - www.biomedcentral.com/bmcbioinformatics/)
Our news journalists report that additional information may be obtained by contacting M. Kruczyk, Dept. of Cell and Molecular Biology, Uppsala University, Husargatan 3, Uppsala, Sweden. Additional authors for this research include H.M. Umer, S. Enroth and J. Komorowski.
Keywords for this news article include: Sweden, Europe, Uppsala, Bioinformatics.
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