By a News Reporter-Staff News Editor at Biotech Week -- Fresh data on Biotechnology are presented in a new report. According to news originating from Chongqing, People's Republic of China, by NewsRx correspondents, research stated, "A new metal-enhanced fluorescence (MEF) based platform was developed on the basis of distance-dependent fluorescence quenching-enhancement effect, which combined the easiness of Ag-thiol chemistry with the MEF property of noble-metal structures as well as the molecular beacon design. For the given sized AgNPs, the fluorescence enhancement factor was found to increase with a d(6) dependency in agreement with fluorescence resonance energy transfer mechanism at shorter distance and decrease with a d(-3) dependency in agreement with plasmonic enhancement mechanism at longer distance between the fluorophore and the AgNP surface."
Our news journalists obtained a quote from the research from Southwest University, "As a proof of concept, the platform was demonstrated by a sensitive detection of mercuric ions, using thymine-containing molecular beacon to tune silver nanoparticle (AgNP)-enhanced fluorescence. Mercuric ions were detected via formation of a thymine-mercuric-thymine structure to open the hairpin, facilitating fluorescence recovery and AgNP enhancement to yield a limit of detection of 1 nM, which is well below the U.S. Environmental Protection Agency regulation of the Maximum Contaminant Level Goal (10 nM) in drinking water."
According to the news editors, the research concluded: "Since the AgNP functioned as not only a quencher to reduce the reagent blank signal but also an enhancement substrate to increase fluorescence of the open hairpin when target mercuric ions were present, the quenching-enhancement strategy can greatly improve the detection sensitivity and can in principle be a universal approach for various targets when combined with molecular beacon design."
For more information on this research see: A distance-dependent metal-enhanced fluorescence sensing platform based on molecular beacon design. Biosensors & Bioelectronics, 2014;52():367-373. Biosensors & Bioelectronics can be contacted at: Elsevier Advanced Technology, Oxford Fulfillment Centre The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England. (Elsevier - www.elsevier.com; Biosensors & Bioelectronics - www.elsevier.com/wps/product/cws_home/405913)
The news correspondents report that additional information may be obtained from Z.P. Zhou, Southwest University, Coll Pharmaceut Sci, Educ Minist, Key Lab Luminescence & Real Time Anal, Chongqing 400715, People's Republic of China. Additional authors for this research include H.D. Huang, Y. Chen, F. Liu, C.Z. Huang and N. Li (see also technology.html">Biotechnology).
Keywords for this news article include: Asia, Biotechnology, Chongqing, People's Republic of China
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