By a News Reporter-Staff News Editor at Health & Medicine Week -- Current study results on Chemiluminescence have been published. According to news originating from Blacksburg, Virginia, by NewsRx correspondents, research stated, "Cost-effective and sensitive aptasensor with guanine chemiluminescence detection capable of simply quantifying thrombin in human serum was developed using thrombin aptamer (TBA), one of the G-quadruplex DNA aptamers, without expensive nanoparticles and complicated procedures. Guanines of G-quadruplex TBA-conjugated carboxyfluorescein (6-FAM) bound with thrombin do not react with 3,4,5-trimethoxylphenylglyoxal (TMPG) in the presence of tetra-n-propylammonium hydroxide (TPA), whereas guanines of free TBA- and TBA-conjugated 6-FAM immobilized on the surface of graphene oxide rapidly react with TMPG to emit light."
Our news journalists obtained a quote from the research from Virginia Polytechnic Institute and State University, "Thus, guanine chemiluminescence in 5% human serum with thrombin was lower than that without thrombin when TBA-conjugated 6-FAM was added in two samples and incubated for 20 min. In other words, the brightness of guanine chemiluminescence was quenched due to the formation of G-quadruplex TBA-conjugated 6-FAM bound with thrombin in a sample. High-energy intermediate, capable of emitting dim light by itself, formed from the reaction between guanines of TBA and TMPG in the presence of TPA, transfers energy to 6-FAM to emit bright light based on the principle of chemiluminescence energy transfer (CRET). G-quadruplex TBA aptasensor devised using the rapid interaction between TBA-conjugated 6-FAM and thrombin quantified trace levels of thrombin without complicated procedures. The limit of detection (LOD=background +3 x standard deviation) of G-quadruplex TBA aptasensor with good linear calibration curve, accuracy, precision, and recovery was as low as 12.3 nM in 5% human serum."
According to the news editors, the research concluded: "Using the technology reported in this research, we expect that various types of G-quadruplex DNA aptasensors capable of specifically sensing a target molecule such as ATP, HIV, ochratoxin, potassium ions, and thrombin can be developed."
For more information on this research see: Rapid and simple G-quadruplex DNA aptasensor with guanine chemiluminescence detection. Biosensors & Bioelectronics, 2014;52():310-316. Biosensors & Bioelectronics can be contacted at: Elsevier Advanced Technology, Oxford Fulfillment Centre The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England. (Elsevier - www.elsevier.com; Biosensors & Bioelectronics - www.elsevier.com/wps/product/cws_home/405913)
The news correspondents report that additional information may be obtained from S. Cho, Virginia Polytechnic Institute & State Univ, Dept. of Wood Sci & Forest Prod, Blacksburg, VA 24061, United States. Additional authors for this research include L. Park, R. Chong, Y.T. Kim and J.H. Lee (see also Chemiluminescence).
Keywords for this news article include: Virginia, Blacksburg, DNA Research, United States, Chemiluminescence, North and Central America
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