By a News Reporter-Staff News Editor at Life Science Weekly -- A new study on Enzymes and Coenzymes is now available. According to news reporting out of Chongqing, People's Republic of China, by NewsRx editors, research stated, "In this work, we described a simple and highly sensitive electrochemiluminescence (ECL) strategy for IgG detection. Firstly, L-cysteine functionalized reduced graphene oxide composite (L-cys-rGO) was decorated on the glassy carbon electrode (GCE) surface."
Our news journalists obtained a quote from the research from the College of Chemistry and Chemical Engineering, "Then anti-IgG was immobilized on the modified electrode surface through the interaction between the carboxylic groups of the L-cys-rGO and the amine groups in anti-IgG. And then biotinylated anti-IgG (bio-anti-IgG) was assembled onto the electrode surface based on the sandwich-type immunoreactions. By the conjunction of biotin and streptavidin (SA), SA was immobilized, which in turn, combined with the biotin labeled initiator strand (S1). In the presence of two single DNA strands of glucose oxidase labeled S2 (GOD-S2) and complementary strand (S3), S1 could trigger the hybridization chain reaction (HCR) among Sl, GOD-S2 and S3. Herein, due to HCR, numerous GOD was efficiently immobilizated on the sensing surface and exhibited excellent catalysis towards glucose to in situ generate amounts of hydrogen peroxide (H2O2), which acted as luminol's co-reactant to significantly enhance the ECL signal. The proposed ECL immunosensor presented predominate stability and high sensibility for determination of IgG in the range from 0.1 pg mL(-1) to 100 ng mL(-1) with a detection limit of 33 fg mL(-1) (S/N=3)."
According to the news editors, the research concluded: "Additionally, the designed ECL immunosensor exhibited a promising application for other protein detection."
For more information on this research see: Amplified electrochemiluminescence of luminol based on hybridization chain reaction and in situ generate co-reactant for highly sensitive immunoassay. Talanta, 2013;115():577-582. Talanta can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Talanta - www.elsevier.com/wps/product/cws_home/525438)
Our news journalists report that additional information may be obtained by contacting L.J. Xiao, Coll Chem & Chem Engn, Educ Minist, Key Lab Luminescence & Real Time Anal, Chongqing 400715, People's Republic of China. Additional authors for this research include Y.Q. Chai, R. Yuan, Y.L. Cao, H.J. Wang and L.J. Bai (see also Enzymes and Coenzymes).
Keywords for this news article include: Asia, Chongqing, Enzymes and Coenzymes, People's Republic of China
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