By a News Reporter-Staff News Editor at Biotech Week -- Fresh data on Biotechnology are presented in a new report. According to news originating from Orono, Maine, by NewsRx correspondents, research stated, "Rapid and specific on-site detection of disease-causing or toxin-producing organisms is essential to public health and safety. Many molecular recognition methods target ribosomal RNA sequences due to their specificity and abundance in the cell."
Our news journalists obtained a quote from the research from the University of Maine, "In this work RNA targets were identified and quantified using a colorimetric bioassay. Peptide nucleic acid (PNA) probes were used to capture RNA targets, and a micrococcal nuclease digestion was performed to remove all non-target nucleic acids, including single base mismatches flanked by adenines or uracils. Perfectly-matched PNA RNA hybrids remained intact and were detected using the symmetrical cyanine dye 3,3'-diethylthiadicarbocyanine iodide (DiSC(2)(5)). Assay applicability to complex samples was demonstrated using mixtures containing RNA sequences from two related, harmful algal bloom-causing Alexandrium species. Target RNA was detected even in mixtures with mismatched sequences in excess of the perfect match. The fieldability of the assay was tested with a portable two-wavelength colorimeter developed to quantify the dye-indicated hybridization signal. The colorimeter sensing performance was shown to be comparable to a laboratory spectrophotometer."
According to the news editors, the research concluded: "This quick, inexpensive and robust system has the potential to replace laborious identification schemes in field environments."
For more information on this research see: A field-deployable colorimetric bioassay for the rapid and specific detection of ribosomal RNA. Biosensors & Bioelectronics, 2014;52():433-437. Biosensors & Bioelectronics can be contacted at: Elsevier Advanced Technology, Oxford Fulfillment Centre The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England. (Elsevier - www.elsevier.com; Biosensors & Bioelectronics - www.elsevier.com/wps/product/cws_home/405913)
The news correspondents report that additional information may be obtained from J. Duy, Univ Maine, Dept. of Mol & Biomed Sci, Orono, ME 04469, United States. Additional authors for this research include R.L. Smith, S.D. Collins and L.B. Connell (see also Biotechnology).
Keywords for this news article include: Biotechnology, Orono, Maine, United States, North and Central America
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