By a News Reporter-Staff News Editor at Biotech Week -- Current study results on Proteins have been published. According to news reporting originating from Sanandaj, Iran, by NewsRx correspondents, research stated, "We have developed a sensitive electrochemical immunosensor for the detection of prostate-specific antigen (PSA), based on covalently immobilizing of anti-PSA and redox mediator (thionine) onto gold nanoparticles-incorporated polyamidoamine dendrimer (AuNPs-PAMAM) and multiwalled carbon nanotubes/ionic liquid/chitosan nanocomposite (MWCNTs/IL/Chit) as the support platform. The MWCNTs/IL/Chit nanocomposite and synthesized AuNPs were characterized using SEM and TEM microscopy techniques."
Our news editors obtained a quote from the research from the University of Kurdistan, "Greatly amplified immunoassay was established by sandwiching the antigen between anti-PSA immobilized on the MWCNTs/IL/Chit/AuNPs-PAMAM interface and anti-PSA labeled with horseradish peroxidase (HRP-labeled anti-PSA) as secondary antibody. Phtaloyl chloride (Ph) was used as linking agent for the subsequent immobilization of AuNPs-PAMAM onto platform and anti-PSA antibody and thionine onto AuNPs-PAMAM dendrimer. The increased electrocatalytic reduction of H2O2 by HRP was monitored by differential pulse voltammetry technique. Under optimized condition the calibration curve for PSA concentration was linear up to 80 ng ml(-1) with detection limit (signal-to-noise ratio of 3) of 1 pg ml(-1). AuNPs-PAMAM dendrimer as platform not only increased the amount of thionine and PSA antibody but also the electron transfer process accelerated by encapsulated AuNPs. Moreover, the proposed PSA immunosensor exhibited excellent stability and reproducibility. Accurate detection of PSA in human serum samples was demonstrated by comparison to standard ELISA assays. In addition, impedance technique was used as simple, rapid, low cost label free analytical method for PSA measurement with detection limit of 0.5 ng ml(-1) at concentration range up to 25 ng ml(-1)."
According to the news editors, the research concluded: "The results indicate that the present protocol is quite promising in developing other electrochemical immunosensors."
For more information on this research see: A highly sensitive prostate-specific antigen immunosensor based on gold nanoparticles/PAMAM dendrimer loaded on MWCNTS/chitosan/ionic liquid nanocomposite. Biosensors & Bioelectronics, 2014;52():20-28. Biosensors & Bioelectronics can be contacted at: Elsevier Advanced Technology, Oxford Fulfillment Centre The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England. (Elsevier - www.elsevier.com; Biosensors & Bioelectronics - www.elsevier.com/wps/product/cws_home/405913)
The news editors report that additional information may be obtained by contacting B. Kavosi, Univ Kurdistan, Res Center Nanotechnol, Sanandaj 6617715175, Iran. Additional authors for this research include A. Salimi, R. Hallaj and K. Amani (see also Proteins).
Keywords for this news article include: Iran, Asia, Antibodies, Sanandaj, Solvents, Chemistry, Immunology, Kallikreins, Ionic Liquids, Blood Proteins, Endopeptidases, Nanotechnology, Electrochemical, Immunoglobulins, Serine Proteases, Neoplasm Antigens, Gold Nanoparticles, Peptide Hydrolases, Emerging Technologies, Biological Tumor Markers
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC