By a News Reporter-Staff News Editor at Life Science Weekly -- Researchers detail new data in Platinum Compounds. According to news reporting out of Ljubljana, Slovenia, by NewsRx editors, research stated, "Conjoint liquid chromatography (CLC) on monolithic convective interaction media (CIM) disks coupled on-line to UV and inductively coupled plasma mass spectrometry (ICP-MS) detectors was used for the first time in speciation analysis of Pt in human serum spiked with Pt-based chemotherapeutics. CIM Protein G and CIM DEAE disks were assembled together in a single housing forming a CLC monolithic column."
Our news journalists obtained a quote from the research from the Institute of Oncology, "Such a set-up allows rapid two-dimensional separation by affinity and ion-exchange (IE) modes to be carried out in a single chromatographic run. By applying isocratic elution with Tris-HCl-NaHCO3 buffer (pH 7.4) in the first minute, followed by gradient elution with 1 mol L-1 NH4Cl (pH 7.4) in the next 9 min, immunoglobulins (IgG) were retained by the Protein G disk enabling subsequent separation of unbound Pt from Pt bound to transferrin (Tf) and albumin (HSA) on the CIM DEAE disk. Further elution with acetic acid (AcOH) in the next 3 min allowed separation of Pt associated with IgG. Separated Pt species were quantified by post-column isotope dilution ICP-MS. Pt recovery on the CLC column was close to 100%. In comparison to commonly applied procedures that involve separation of protein peaks by size-exclusion chromatography (SEC) followed by IE separation of metal-based chemotherapeutic fractions bound to serum proteins, the CLC method developed is much faster and simpler. Its sensitivity (LOQs adequate for quantification of all separated Pt species, lower than 2.4 ng Pt mL(-1)), good selectivity and method repeatability (RSD +/- 3%) enabled investigation of the kinetics of interaction of Pt-based chemotherapeutics with serum proteins and the distribution of Pt species in spiked human serum. Pt species present in spiked serum were bound preferentially to HSA. The proportion of Pt associated with IgG and Tf was lower than 13%. Cisplatin and especially oxaliplatin react rapidly with serum proteins, while carboplatin much less."
According to the news editors, the research concluded: "The method developed may be reliably applied in preclinical and clinical studies of the kinetics of the interaction and distribution of different metallodrugs with proteins in blood serum."
For more information on this research see: A novel method for speciation of Pt in human serum incubated with cisplatin, oxaliplatin and carboplatin by conjoint liquid chromatography on monolithic disks with UV and ICP-MS detection. Talanta, 2013;116():141-148. Talanta can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Talanta - www.elsevier.com/wps/product/cws_home/525438)
Our news journalists report that additional information may be obtained by contacting A. Martincic, Inst Oncol Ljubljana, Dept. of Radiotherapy, SI-1000 Ljubljana, Slovenia. Additional authors for this research include M. Cemazar, G. Sersa, V. Kovac, R. Milacic and J. Scancar (see also Platinum Compounds).
Keywords for this news article include: Europe, Slovenia, Peptides, Proteins, Ljubljana, Cisplatin, Amino Acids, Carboplatin, Chlorine Compounds, Nitrogen Compounds, Organoplatinum Compounds
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC