By a News Reporter-Staff News Editor at Biotech Week -- Investigators discuss new findings in Gold Nanoparticles. According to news reporting from Beijing, People's Republic of China, by NewsRx journalists, research stated, "Gold nanoparticles (AuNPs) based fluorescence quenching or colorimetric aptasensor have been developed for many analytes recently largely because of the ease of detection, high sensitivity, and potential for high-throughput analysis. However, the effects of remnant non-AuNPs components in the colloid gold solution on these assays performance remain unclear."
The news correspondents obtained a quote from the research from the Ministry of Agriculture, "For the first time, we demonstrated that the remnant sodium citrate and the reaction products of three acids play counteractive roles in AuNPs based fluorescence quenching and colorimetric aptasensor in three ways in this study. First, the remnant sodium citrate in the colloid gold solution could increase the fluorescence intensity of FAM labeled on the aptamer that reduce the efficiency of AuNPs fluorescent quenching. Second, the reaction products of citric acid, HCl and ketoglutaric acid reduce the fluorescence recovery by quenching the fluorescence of FAM labeled on the aptamer dissociated from the surface of AuNPs upon addition of target. Lastly, the reaction products of three acids reduce the pH value of the colloid gold solution that reduce the sensitivity of AuNPs based colorimetric aptasensor by increasing the adsorption of aptamer to surface of AuNPs. With sulfadimethoxine and thrombin as model analytes, we found that water resuspended AuNPs can significantly increase the sensitivity by more than 10-fold for AuNPs based fluorescence quenching aptasensor. In the AuNPs based colorimetric aptasensor for sulfadimethoxine using the water resuspended AuNPs, the sensitivity also was increased by 10-fold compared with that of original AuNPs."
According to the news reporters, the research concluded: "The findings in this study provide theoretical guidance for further improving AuNPs based fluorescent quenching and colorimetric aptasensor by adjusting the composition of AuNPs solution."
For more information on this research see: Improving sensitivity of gold nanoparticle based fluorescence quenching and colorimetric aptasensor by using water resuspended gold nanoparticle. Biosensors & Bioelectronics, 2014;52():265-270. Biosensors & Bioelectronics can be contacted at: Elsevier Advanced Technology, Oxford Fulfillment Centre The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England. (Elsevier - www.elsevier.com; Biosensors & Bioelectronics - www.elsevier.com/wps/product/cws_home/405913)
Our news journalists report that additional information may be obtained by contacting J.C. Liu, Minist Agr, Key Lab Agri Food Qual & Safety, Beijing 100081, People's Republic of China. Additional authors for this research include Z. Guan, Z.Z. Lv, X.L. Jiang, S.M. Yang and A.L. Chen (see also Gold Nanoparticles).
Keywords for this news article include: Asia, Beijing, Nanotechnology, Gold Nanoparticles, Emerging Technologies, People's Republic of China
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