By a News Reporter-Staff News Editor at Gene Therapy Weekly -- Researchers detail new data in Biochemistry. According to news reporting originating in Seattle, Washington, by NewsRx journalists, research stated, "5-HT1B receptors are densely expressed on terminals of medium spiny neurons projecting from the nucleus accumbens shell (NAccSh) to the ventral tegmental area, where 5-HT1B receptors modulate GABA release directly, and firing of dopaminergic neurons indirectly. While interactions between NAccSh 5-HT1B receptors and stress have been reported in early stages of psychostimulant-induced neuroadaptations, specifically psychomotor sensitization, the effect of this interaction on later stages of drug seeking is currently unknown."
The news reporters obtained a quote from the research from the University of Washington, "Here, we examined the effect of herpes simplex virus (HSV)-mediated overexpression of NAccSh 5-HT1B receptors on reinstatement of cocaine seeking induced by exposure to stress or a cocaine prime. Rats were trained to self-administer cocaine (0.75 mg/kg/infusion) and the operant response was extinguished. Rats were then injected with viral vector expressing 5-HT1B and green fluorescent protein (GFP) or GFP alone into the NAccSh. The effect of 5-HT1B receptor overexpression was assessed on reinstatement induced by intermittent footshock (0.5 mA for 15 min) or a cocaine prime (10 mg/kg, ip). NAccSh 5-HT1B receptor overexpression had no effect on footshock reinstatement while significantly decreasing cocaine priming-induced reinstatement. We also found that NAccSh overexpression of 5-HT1B receptors had no effect on saccharin intake following social defeat stress. These results suggest that the efficacy of pharmacological agents targeting 5-HT1B receptors for the treatment of cocaine relapse will depend largely on the nature of the reinstating stimulus."
According to the news reporters, the research concluded: "Taken together with previous results, it appears that NAccSh 5-HT1B receptors influence stress responses in early, but not in the later stages of psychostimulant-induced neuroadaptations."
For more information on this research see: Differential effect of viral overexpression of nucleus accumbens shell 5-HT1B receptors on stress- and cocaine priming-induced reinstatement of cocaine seeking. Pharmacology Biochemistry and Behavior, 2013;112():89-95. Pharmacology Biochemistry and Behavior can be contacted at: Pergamon-Elsevier Science Ltd, The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, England. (Elsevier - www.elsevier.com; Pharmacology Biochemistry and Behavior - www.elsevier.com/wps/product/cws_home/525485)
Our news correspondents report that additional information may be obtained by contacting S.G. Nair, University of Washington, Dept. of Psychiat & Behav Sci, Harborview Med Center, Seattle, WA 98104, United States. Additional authors for this research include A.R. Furay, Y.S. Liu and J.F. Neumaier (see also Biochemistry).
Keywords for this news article include: Biotechnology, Seattle, Washington, Biochemistry, Gene Therapy, United States, Bioengineering, North and Central America
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