By a News Reporter-Staff News Editor at Drug Week -- A new study on Fibroblasts is now available. According to news reporting originating in Harbin, People's Republic of China, by NewsRx journalists, research stated, "Previously we have identified a series of cellular miRNA molecules up- or down-regulated in infectious bursal disease virus (IBDV) infected chicken embryo fibroblasts and Bursa of Fabricius with gene microarray analysis. Here we studied in detail a relatively well studied miRNA, gga-miR-21, for better understanding miRNAs involvement in IBDV-host interactions."
The news reporters obtained a quote from the research from Harbin Veterinary Research Institute, "Chicken pri-gga-miRNA-21 and a control miRNA Caenorhabditis elegans pri-cel-lin-4 gene were cloned into a lentiviral vector, respectively. The resulting recombinant lentiviruses were used to infect chicken fibroblast cell line DF-1, and two stable cell lines, DF-miR-21 (overexpressing gga-miR-21) and DF-lin-4 (overexpressing cel-lin-4), were selected. Replication of IBDV in DF-miR-21, DF-lin-4 and DF-1 cells were compared and molecular mechanism of IBDV replication alteration was explored using bioinformatics, reporter gene system, qRT-PCR and Western blot analysis. IBDV replication was markedly lower in DF-miR-21 than in DF-lin-4 or DF-1 cells. A gga-miR-21 target sequence was identified within IBDV VP1 gene (1713-1734 bp). Fusion of a 520nt long partial IBDV VP1 gene containing the target with a luciferase gene resulted in significantly lower transient luciferase activity in DF-miR-21 cells as compared to that in DF-lin-4 or DF-1 cell. Following IBDV infection of the cell lines, VP1 protein level in DF-miR-21 cells was dramatically lower than that in DF-lin-4 or DF-1 cells but VP1 mRNA level was not different."
According to the news reporters, the research concluded: "The finding indicated that gga-miR-21 could suppress IBDV replication through down regulating IBDV VP1 expression at translational level."
For more information on this research see: Overexpression of microRNA gga-miR-21 in chicken fibroblasts suppresses replication of infectious bursal disease virus through inhibiting VP1 translation. Antiviral Research, 2013;100(1):196-201. Antiviral Research can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Antiviral Research - www.elsevier.com/wps/product/cws_home/521852)
Our news correspondents report that additional information may be obtained by contacting Y.S. Wang, Chinese Academy Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Harbin 150001, People's Republic of China. Additional authors for this research include W. Ouyang, Q.X. Pan, X.L. Wang, X.X. Xia, Z.W. Bi, Y.Q. Wang and X.M. Wang (see also Fibroblasts).
Keywords for this news article include: Asia, Harbin, Virology, Fibroblasts, RNA Viruses, Birnaviridae, Avibirnavirus, Connective Tissue Cells, People's Republic of China, Infectious Bursal Disease Virus
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