By a News Reporter-Staff News Editor at Agriculture Week -- Data detailed on Food Science have been presented. According to news reporting out of Ljubljana, Slovenia, by VerticalNews editors, research stated, "A rapid, reliable, and sensitive loop-mediated isothermal amplification (LAMP) system was developed for screening of genetically modified organisms (GMOs). The optimized LAMP assays using designed primers target commonly employed promoters, i.e., Cauliflower Mosaic Virus 35S (P-35S) and Figwort Mosaic Virus promoter (P-FMV), and marker genes, i.e., aminoglycoside 3'-adenyltransferase (aadA), neomycin phosphotransferase II (nptII), and beta-glucuronidase (uidA)."
Our news journalists obtained a quote from the research from the National Institute of Biology, "The specificity and performance of the end-point and real-time LAMP assays were confirmed using eight genetically modified (GM) cotton events on four detection systems, employing two chemistries. LAMP assays on the isothermal real-time system were found to be most sensitive, detecting up to four target copies, within 35 min. The LAMP assays herein presented using alternate detection systems can be effectively utilized for rapid and cost-effective screening of the GM status of a sample, irrespective of the crop species or GM trait."
According to the news editors, the research concluded: "These assays coupled with a fast and simple DNA extraction method may further facilitate on-site GMO screening."
For more information on this research see: Loop-Mediated Isothermal Amplification: Rapid Visual and Real-Time Methods for Detection of Genetically Modified Crops. Journal of Agricultural and Food Chemistry, 2013;61(47):11338-11346. Journal of Agricultural and Food Chemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Journal of Agricultural and Food Chemistry - www.pubs.acs.org/journal/jafcau)
Our news journalists report that additional information may be obtained by contacting G.J. Randhawa, Natl Inst Biol, Dept. of Biotechnol & Syst Biol, SI-1000 Ljubljana, Slovenia. Additional authors for this research include M. Singh, D. Morisset, P. Sood and J. Zel.
Keywords for this news article include: Europe, Slovenia, Ljubljana, Food Science
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