By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Proteins have been published. According to news reporting out of Nantou, Taiwan, by NewsRx editors, research stated, "This paper presents the use of fluorescent biofunctional nanoparticles (10-30 nm) to detect alpha-fetoprotein (AFP) in a thin-channel magnetic immunoassay. We used an AFP model biomarker and s-shaped deposition zones to test the proposed detection method."
Our news journalists obtained a quote from the research from National Chi Nan University, "The results show that the detection using fluorescent biofunctional nanoparticle has a higher throughput than that of functional microparticle used in previous experiments on affinity reactions. The proposed method takes about 3 min (versus 150 min of previous method) to detect 100 samples. The proposed method is useful for screening biomarkers in clinical applications, and can reduce the run time for sandwich immunoassays to less than 20 min. The detection limits (0.06 pg/ml) and linear ranges (0.068 pg/ml-0.68 ng/ml) of AFP using fluorescent biofunctional nanoparticles are the same as those of using functional microparticles within experimental errors. This detection limit is substantially lower and the linear range is considerably wider than those of enzyme-linked immunosorbent assay (ELISA) and other methods in sandwich immunoassay methods."
According to the news editors, the research concluded: "The differences between this method and an ELISA in AFP measurements of serum samples were less than 12 %. The proposed method provides simple, fast, and sensitive detection with a high throughput for biomarkers."
For more information on this research see: Detection of alpha-fetoprotein in magnetic immunoassay of thin channels using biofunctional nanoparticles. Journal of Nanoparticle Research, 2013;16(1):1-8. Journal of Nanoparticle Research can be contacted at: Springer, Van Godewijckstraat 30, 3311 Gz Dordrecht, Netherlands. (Springer - www.springer.com; Journal of Nanoparticle Research - www.springerlink.com/content/1388-0764/)
Our news journalists report that additional information may be obtained by contacting H.Y. Tsai, Natl Chi Nan Univ, Dept. of Appl Chem, Puli 545, Nantou, Taiwan. Additional authors for this research include B.Z. Gao, S.F. Yang, C.S. Li and C.B. Fuh (see also Proteins).
Keywords for this news article include: Asia, Nantou, Taiwan, Nanoparticle, Fetal Proteins, Nanotechnology, alpha-Fetoproteins, Emerging Technologies, Biological Tumor Markers
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