By a News Reporter-Staff News Editor at Cancer Gene Therapy Week -- Researchers detail new data in Biotechnology. According to news reporting originating from Jinzhou, People's Republic of China, by NewsRx correspondents, research stated, "In this study, pH-sensitive biomaterials coated polymer/DNA nanocomplexes containing a high mobility group box 1 (HMGB1) were developed as an efficient non-viral gene delivery system. HMGB 1 is a family of endogenous molecules that contains nuclear locating sequences (NSL)."
Our news editors obtained a quote from the research from Liaoning Medical University, "Polyethylene glycol tethered carboxylated chitosan modified with folic acid (FA-PEG-CCTS) was synthesized and its buffering capacity was determined by acid-base titration. A pH-sensitive core-shell system FA-PEG-CCTS/PAMAM/HMGB1/pDNA nanocomplexes (FPCPHDs), was prepared and characterized. Electrophoresis showed that FPCPHDs were resistant to heparin replacement and DNase I digestion. FPCPHDs exhibited only minor toxic effects on HepG2 and KB cells. The results of both luciferase activity assay and RFP fluorescence intensity analysis showed that FPCPHDs enhanced gene transfection and expression in KB cells. Moreover, gene transfection and expression in KB cells were inhibited by free folic acid. Intracellular trafficking of FPCPHDs in KB cells showed that FPCPHDs could rapidly escape from endo-lysosomes and become exclusively located in the nucleus at 3 h post transfection. In addition, FPCPHDs exhibited increased red fluorescence protein (RFP) expression at the tumor site of S180 xenograft nude mice."
According to the news editors, the research concluded: "All results suggest that FPCPHDs is an efficient approach to improve the transfection and expression efficiency in most FR-positive cancer cells."
For more information on this research see: A pH-sensitive gene delivery system based on folic acid-PEG-chitosan - PAMAM-plasmid DNA complexes for cancer cell targeting. Biomaterials, 2013;34(38):10120-10132. Biomaterials can be contacted at: Elsevier Sci Ltd, The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England. (Elsevier - www.elsevier.com; Biomaterials - www.elsevier.com/wps/product/cws_home/30392)
The news editors report that additional information may be obtained by contacting M.Y. Wang, Liaoning Med Univ, Sch Pharm, Jinzhou 121001, People's Republic of China. Additional authors for this research include H.Y. Hu, Y.Q. Sun, L.P. Qiu, J. Zhang, G.N. Guan, X.L. Zhao, M.X. Qiao, L. Cheng, L.F. Cheng and D.W. Chen (see also Biotechnology).
Keywords for this news article include: Asia, Biotechnology, Jinzhou, KB Cells, Oncology, Cell Line, Hela Cells, DNA Research, Bioengineering, Cancer Gene Therapy, People's Republic of China
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