By a News Reporter-Staff News Editor at Life Science Weekly -- New research on Proteins is the subject of a report. According to news reporting from Chongqing, People's Republic of China, by NewsRx journalists, research stated, "In this research, a novel electrochemical immunosensor for competitive detection of pregnancy-associated plasma protein-A (PAPP-A) in serum had been successfully developed. The PAPP-A was covalently immobilized on the glass carbon electrode (GCE) modified with functional single walled carbon nanotubes/chitosan (SWNTs/CS) to amplify signal."
The news correspondents obtained a quote from the research from Chongqing Medical University, "After the modified GCE was incubated with the intermixture of biotin-anti-PAPP-A antibody and samples PAPP-A, the active streptavidin-alkaline phosphatase (ST-AP) bonded with the remaining biotin-anti-PAPP-A to catalyze the hydrolysis of the enzyme substrate alpha-NP, then the hydrolysis product could be oxidized to produce the differential pulse voltammetric (DPV) signal. The DPV current decreased with the increasing PAPP-A concentration. Meanwhile, the functionalization of the immunosensor was characterized with three electrochemical techniques: scanning electron microscope (SEM), electrochemical impedance spectroscopic (EIS) and cyclic voltammetric (CV). Under optimal conditions, a good linear relationship between the concentrations of the PAPP-A and DPV currents was achieved in the range from 0.05 to 10 mu g mL(-1) with the detection limit of 39 ng mL(-1), the reproducibility was calculated to be 3.39% and 8.60% respectively, and a good selectivity was obtained for the developed immunosensors."
According to the news reporters, the research concluded: "Finally, this method was successfully applied for direct quantitative detection of complex clinical serum specimens and the results were in good agreement with the time-resolved immunofluorometric assay (TrIFMA), which makes it be a promising alternative for the clinical detection of PAPP-A in the future."
For more information on this research see: Competitive detection of pregnancy-associated plasma protein-A in serum using functional single walled carbon nanotubes/chitosan-based electrochemical immunosensor. Journal of Electroanalytical Chemistry, 2013;708():95-100. Journal of Electroanalytical Chemistry can be contacted at: Elsevier Science Sa, PO Box 564, 1001 Lausanne, Switzerland (see also Proteins).
Our news journalists report that additional information may be obtained by contacting J.X. Li, Chongqing Med Univ, Affiliated Hosp 1, Mol Oncol & Epigenet Lab, Chongqing 400016, People's Republic of China. Additional authors for this research include G. Liu, W. Zhang, W. Cheng, H.B. Xu and S.J. Ding.
Keywords for this news article include: Asia, Chongqing, Chemistry, Fullerenes, Nanotechnology, Electrochemical, Peptide Hydrolases, Pregnancy Proteins, Emerging Technologies, Enzymes and Coenzymes, Metalloendopeptidases, People's Republic of China, Single Walled Carbon Nanotubes, Pregnancy-Associated Plasma Protein-A
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