By a News Reporter-Staff News Editor at Biotech Week -- Investigators publish new report on DNA Research. According to news reporting originating from Knoxville, Tennessee, by NewsRx correspondents, research stated, "Genetically engineered (GE) ringspot virus-resistant papaya cultivars 'Rainbow' and 'SunUp' have been grown in Hawai'i for over 10 years. In Hawai'i, the introduction of GE papayas into regions where non-GE cultivars are grown and where feral non-GE papayas exist have been accompanied with concerns associated with transgene flow."
Our news editors obtained a quote from the research from the University of Tennessee, "Of particular concern is the possibility of transgenic seeds being found in non-GE papaya fruits via cross-pollination. Development of high-throughput methods to reliably detect the adventitious presence of such transgenic material would benefit both the scientific and regulatory communities. We assessed the accuracy of using conventional qualitative polymerase chain reaction (PCR) as well as real-time PCR-based assays to quantify the presence of transgenic DNA from bulk samples of non-GE papaya seeds. In this study, an optimized method of extracting high quality DNA from dry seeds of papaya was standardized. A reliable, sensitive real-time PCR method for detecting and quantifying viral coat protein (cp) transgenes in bulk seed samples utilizing the endogenous papain gene is presented. Quantification range was from 0.01 to 100 ng/?l of GE-papaya DNA template with a detection limit as low as 0.01% (10 pg). To test this system, we simulated transgene flow using known quantities of GE and non-GE DNA and determined that 0.038% (38 pg) GE papaya DNA could be detected using real-time PCR. We also validated this system by extracting DNA from known ratios of GE seeds to non-GE seeds of papaya followed by real-time PCR detection and observed a reliable detection limit of 0.4%. This method for the quick and sensitive detection of transgenes in bulked papaya seed lots using conventional as well as real-time PCR-based methods will benefit numerous stakeholders. In particular, this method could be utilized to screen selected fruits from maternal non-GE papaya trees in Hawai'i for the presence of transgenic seed at typical regulatory threshold levels."
According to the news editors, the research concluded: "Incorporation of subtle differences in primers and probes for variations in cp worldwide should allow this method to be utilized elsewhere when and if deregulation of transgenic papaya occurs."
For more information on this research see: Sensitivity of a real-time PCR method for the detection of transgenes in a mixture of transgenic and non-transgenic seeds of papaya (Carica papaya L.). Bmc Biotechnology, 2013;13():69. (BioMed Central - www.biomedcentral.com/; Bmc Biotechnology - www.biomedcentral.com/bmcbiotechnol/)
The news editors report that additional information may be obtained by contacting M. Nageswara-Rao, Dept. of Plant Sciences, The University of Tennessee, 252 Ellington Plant Sciences, 2431 Joe Johnson Dr, Knoxville, TN 37996, United States. Additional authors for this research include C. Kwit, S. Agarwal, M.T. Patton, J.A. Skeen, J.S. Yuan, R.M. Manshardt and C.N Stewart (see also DNA Research).
Keywords for this news article include: Knoxville, Tennessee, Viral DNA, DNA Research, United States, North and Central America.
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