By a News Reporter-Staff News Editor at Clinical Trials Week -- Data detailed on Stem Cell Research have been presented. According to news reporting originating from Tehran, Iran, by NewsRx correspondents, research stated, "To increase the understanding of the applicability of biomaterials and growth factors in enhancing stem cell-based bone regeneration modalities, this study evaluated the effects of enamel matrix derivative (EMD) and recombinant human transforming growth factor-beta (rhTGF-beta) on osteoblastic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) as well as human periodontal ligament stem cells (hPDLSCs). hBMSCs and hPDLSCs were obtained, and identification of stem cell surface markers was performed according to the criteria of the International Society for Cellular Therapy."
Our news editors obtained a quote from the research from the Shaheed Beheshti University of Medical Sciences, "Each group of stem cells was separately treated with a serial dilution of EMD (10, 50, and 100 mu g/mL) or rhTGF-beta (10 ng/mL). Osteoblastic differentiation was examined through in vitro matrix mineralization by alizarin red staining, and mRNA expression of osteopontin and osteonectin was determined by quantitative reverse-transcriptase polymerase chain reaction. hPDLSCs were further assessed for osteocalcin mRNA expression. Stem cells cultured in osteogenic medium were employed as a standard positive control group. In none of the experimental groups were bone-related mRNAs detected subsequent to treatment with EMD for 5, 10, and 15 days. Alizarin red staining on day 21 was negative in EMD-treated BMSC and PDLSC cultures. In rhTGF-beta-supplemented BMSC culture, expression of osteonectin mRNA was demonstrated on day 15, which was statistically comparable to the positive control group. Nevertheless, extracellular matrix mineralization was inhibited in both groups of stem cells. Within the limitations of this study, it could be concluded that EMD with a concentration of 10, 50, or 100 mu g/mL has no appreciable effect on osteoblastic differentiation of BMSCs and PDLSCs. Application of rhTGF-beta increased osteonectin mRNA expression in BMSCs."
According to the news editors, the research concluded: "This finding corroborates the hypothesis that TGF-beta might be involved in early osteoblastic maturation."
For more information on this research see: Osteoblastic Differentiation of Human Stem Cells Derived from Bone Marrow and Periodontal Ligament Under the Effect of Enamel Matrix Derivative and Transforming Growth Factor-Beta. International Journal of Oral & Maxillofacial Implants, 2013;28(6):E440-E450. International Journal of Oral & Maxillofacial Implants can be contacted at: Quintessence Publishing Co Inc, 4350 Chandler Drive, Hanover Park, IL 60133, USA (see also Stem Cell Research).
The news editors report that additional information may be obtained by contacting B. Houshmand, Shahid Beheshti Univ Med Sci, Dental Res Center, Dept. of Oral & Maxillofacial Surg, Fac Dental, Tehran, Iran. Additional authors for this research include H. Behnia, A. Khoshzaban, G. Morad, G. Behrouzi, S.G. Dashti and A. Khojasteh.
Keywords for this news article include: Iran, Asia, Tissue Engineering, Biomedical Engineering, Biomedicine, Tehran, Cytokines, Bone Marrow, Osteoblasts, Osteonectin, Bone Research, Glycoproteins, Immune System, Bioengineering, Bone Regeneration, Stem Cell Research, Regeneration Medicine, Connective Tissue Cells, Calcium-Binding Proteins
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