By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on DNA Research. According to news originating from Novosibirsk, Russia, by NewsRx correspondents, research stated, "Quantum dots are semiconductor nanocrystals, having very useful luminescent properties. We synthesized cadmium sulfide nanocrystals with infinite solubility and long-time stability in water by using the thioglycolic acid as capping agent and Tris-base as counter ion."
Our news journalists obtained a quote from the research from Novosibirsk State University, "The obtained nanoparticles in the concentration range up to 200 nM did not influence on the activity of DNA polymerase beta in reverse micelles. The primed M13mp19 DNA upon the irradiation of nanoparticles with UV-light at 312 nm can absorb them. We obtained and characterized by photon correlation spectroscopy the covalent conjugates of DNA polymerase and CdS quantum dots. The conjugates sustained enzymatic activity; however, two-fold decrease in activity of the enzyme was observed. Using DNA template as a ruler and the activity of DNA polymerase, we estimated the Forster distance (2.3 nm) for CdS nanoparticles (donor) and 5(6)-carboxytetramethylrhodamine (acceptor) in the Forster resonance energy transfer (FRET). The conjugate we obtained is functional enzyme, opening the possibility of using this nanohybrid in DNA repair study, for example, to track DNA polymerase beta on DNA within DNA repair machine."
According to the news editors, the research concluded: "The proposed experimental protocols are optimized for a convenient, economic and practical use by many researchers."
For more information on this research see: Synthesis of the conjugates of DNA polymerase beta with cadmium sulfide nanoparticles and their activity in reverse micelles. Journal of Molecular Catalysis B-Enzymatic, 2013;98():46-54. Journal of Molecular Catalysis B-Enzymatic can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands (see also DNA Research).
The news correspondents report that additional information may be obtained from R.O. Anarbaev, Novosibirsk State Univ, Novosibirsk 630090, Russia. Additional authors for this research include I.A. Vasil'eva and O.I. Lavrik.
Keywords for this news article include: Russia, Eurasia, Cadmium, Polymerase, Novosibirsk, Nanocrystal, DNA Research, Nanoparticle, Quantum Dots, Nanotechnology, Quantum Physics, Transition Elements, Emerging Technologies, Enzymes and Coenzymes
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