By a News Reporter-Staff News Editor at Biotech Week -- Current study results on Peptide Proteins have been published. According to news originating from Madrid, Spain, by NewsRx correspondents, research stated, "An amperometric immunosensor for the determination of the hormone insulin-like growth factor 1 (IGF1) is reported for the first time in this work. As electrochemical transducer, a multiwalled carbon nanotubes-modified glassy carbon electrode on which poly(pyrrole propionic acid) was electropolymerized was prepared."
Our news journalists obtained a quote from the research from Complutense University, "This approach provided a high content of surface confined carboxyl groups suitable for direct covalent binding of anti-IGF1 monoclonal antibody. A sandwich-type immunoassay using a polyclonal antibody labeled with peroxidase, hydrogen peroxide as the enzyme substrate and catechol as redox mediator was employed to monitor the affinity reaction. All the variables involved in the preparation of the modified electrode were optimized and the electrodes were characterized by electrochemical impedance spectroscopy and cyclic voltammetry. Moreover, the different experimental variables affecting the amperometric response of the immunosensor were also optimized. The calibration graph for IGF1 showed a range of linearity extending from 0.5 to 1000 pg/mL, with a detection limit, 025 pg/mL, more than 100 times lower than the lowest values reported for the ELISA immunoassays available for IGF1 (30 pg/mL, approximately). Excellent reproducibility for the measurements carried out with different immunosensors and selectivity against other hormones were also evidenced."
According to the news editors, the research concluded: "A commercial human serum spiked with IGF1 at different levels between 0.01 and 10.0 ng/mL was analyzed with good results."
For more information on this research see: Electrochemical immunosensor for the determination of insulin-like growth factor-1 using electrodes modified with carbon nanotubes-poly(pyrrole propionic acid) hybrids. Biosensors & Bioelectronics, 2014;52():98-104. Biosensors & Bioelectronics can be contacted at: Elsevier Advanced Technology, Oxford Fulfillment Centre The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England. (Elsevier - www.elsevier.com; Biosensors & Bioelectronics - www.elsevier.com/wps/product/cws_home/405913)
The news correspondents report that additional information may be obtained from V. Serafin, Complutense University Madrid, Fac Chem, Dept. of Analyt Chem, E-28040 Madrid, Spain. Additional authors for this research include L. Agui, P. Yanez-Sedeno and J.M. Pingarron (see also Peptide Proteins).
Keywords for this news article include: Antibodies, Spain, Madrid, Europe, Chemistry, Fullerenes, Immunology, Proinsulin, Propionates, Blood Proteins, Nanotechnology, Electrochemical, Immunoglobulins, Propionic Acids, Carbon Nanotubes, Peptide Hormones, Peptide Proteins, Emerging Technologies
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