By a News Reporter-Staff News Editor at Ecology, Environment & Conservation -- Current study results on Environmental Water Research have been published. According to news reporting originating from St. Paul, Minnesota, by VerticalNews correspondents, research stated, "Molecular microbial community analyses provide information on thousands of microorganisms simultaneously, and integrate biotic and abiotic perturbations caused by fecal contamination entering water bodies. A few studies have explored community methods as emerging approaches for microbial source tracking (MST), however, an evaluation of the current state of this approach is lacking."
Our news editors obtained a quote from the research from the University of Minnesota, "Here, we utilized three types of community-based methods with 64 blind, single- or dual-source, challenge samples generated from 12 sources, including: humans (feces), sewage, septage, dogs, pigs, deer, horses, cows, chickens, gulls, pigeons, and geese. Each source was a composite from multiple donors from four representative geographical regions in California. Methods evaluated included terminal restriction fragment polymorphism (TRFLP), phylogenetic microarray (PhyloChip), and next generation (Illumina) sequencing. These methods correctly identified dominant (or sole) sources in over 90% of the challenge samples, and exhibited excellent specificity regardless of source, rarely detecting a source that was not present in the challenge sample. Sensitivity, however, varied with source and community analysis method. All three methods distinguished septage from human feces and sewage, and identified deer and horse with 100% sensitivity and 100% specificity. Method performance improved if the composition of blind dual-source reference samples were defined by DNA contribution of each single source within the mixture, instead of by Enterococcus colony forming units. Data analysis approach also influenced method performance, indicating the need to standardize data interpretation."
According to the news editors, the research concluded: "Overall, results of this study indicate that community analysis methods hold great promise as they may be used to identify any source, and they are particularly useful for sources that currently do not have, and may never have, a source-specific single marker gene."
For more information on this research see: Evaluation of molecular community analysis methods for discerning fecal sources and human waste. Water Research, 2013;47(18):6862-6872. Water Research can be contacted at: Pergamon-Elsevier Science Ltd, The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, England. (Elsevier - www.elsevier.com; Water Research - www.elsevier.com/wps/product/cws_home/309)
The news editors report that additional information may be obtained by contacting Y.P. Cao, University of Minnesota, Inst Biotechnol, St Paul, MN 55108, United States. Additional authors for this research include L.C. Van De Werfhorst, E.A. Dubinsky, B.D. Badgley, M.J. Sadowsky, G.L. Andersen, J.F. Griffith and P.A. Holden.
Keywords for this news article include: St. Paul, Minnesota, United States, North and Central America, Environmental Water Research
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