By a News Reporter-Staff News Editor at Biotech Week -- Investigators discuss new findings in Immunology. According to news reporting originating in Genoa, Italy, by NewsRx journalists, research stated, "A nanostructured electrochemical biosensor for detecting proteins of interest in work of art, in particular in tempera paintings, is presented. To determine egg yolk we focus here on the determination of immunoglobulin IgY."
The news reporters obtained a quote from the research from the University of Genoa, "The transducers are nanoelectrode ensembles (NEEs), prepared via membrane templated electroless deposition of gold. Because of their geometrical and diffusion characteristics, NEEs are characterized by significantly low detection limits, moreover they display the capability of capturing proteins by interaction with the polycarbonate membrane of the NEE. At first, the proteic component of the paint is extracted by ultrasonication in an aqueous buffer, then IgY is captured by incubation on the NEE. The immunoglobulin is detected by treatment with anti-IgY labeled with horse radish peroxidase (Anti-IgY-HRP). The binding of the Anti-IgY-HRP is detected by recording the electrocatalytic signal caused by addition of H2O2 and methylene blue. The sensor detection capabilities are tested by analyzing both paint models, prepared in the lab, and real samples, from paintings of the XVIII-XX(century."
According to the news reporters, the research concluded: "Multivariate exploratory analysis is applied to classify the voltammetric patterns, confirming the capability to differentiate egg-yolk tempera from other kind of tempera binders as well as from acrylic or oil paints."
For more information on this research see: Electrochemical immunosensor based on ensemble of nanoelectrodes for immunoglobulin IgY detection: Application to identify hen's egg yolk in tempera paintings. Biosensors & Bioelectronics, 2014;52():403-410. Biosensors & Bioelectronics can be contacted at: Elsevier Advanced Technology, Oxford Fulfillment Centre The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England. (Elsevier - www.elsevier.com; Biosensors & Bioelectronics - www.elsevier.com/wps/product/cws_home/405913)
Our news correspondents report that additional information may be obtained by contacting F. Bottari, University of Genoa, Dept. of Pharm, I-16147 Genoa, Italy. Additional authors for this research include P. Oliveri and P. Ugo (see also Immunology).
Keywords for this news article include: Genoa, Italy, Europe, Chemistry, Immunology, Immunoproteins, Electrochemical, Immunoglobulins, Serum Globulins
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC