By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators discuss new findings in Proteins. According to news reporting out of Harbin, People's Republic of China, by NewsRx editors, research stated, "To construct a scaffold using silk fibroin (SF) and chitosan (CS) that could replace the corneal stroma with the biological characteristics of the scaffold materials still intact. To develop an organotypic corneal stroma, SF and CS were chosen to synthesise the tissue-engineered bioscaffold."
Our news journalists obtained a quote from the research from Harbin Medical University, "We cultured primary rabbit corneal epithelial cells and corneal stromal cells in vitro. Keratocytes were used to assess cytotoxicity on SF-CS (SFCS) blends, which was determined by a Cell Counting Kit-8 assay. The corneal lamellar scaffolds were developed with sequential culture techniques to form cell-scaffold constructs. Implantation was tested in 15 New Zealand White rabbits. The corneal substitutes were analysed by light and electron microscopy. The reconstructed lamellar cornea was comparable to native tissue, with high levels of K3/12 expression in the corneal epithelial cells and vimentin in the stromal cells; moreover, the morphology and the position of the cells could be distinguished by histological methods. There was no sign of any immune reaction in or around the transplanted discs 12 weeks after implantation."
According to the news editors, the research concluded: "A SFCS scaffold might be a suitable blend for corneal tissue engineering."
For more information on this research see: Use of a Silk Fibroin-Chitosan Scaffold to Construct a Tissue-Engineered Corneal Stroma. Cells Tissues Organs, 2013;198(3):190-197. Cells Tissues Organs can be contacted at: Karger, Allschwilerstrasse 10, Ch-4009 Basel, Switzerland. (Karger - www.karger.com/; Cells Tissues Organs - content.karger.com/ProdukteDB/produkte.asp?Aktion=JournalHome&ProduktNr=224197)
Our news journalists report that additional information may be obtained by contacting L. Guan, Harbin Med Univ, Affiliated Hosp 1, Hosp Eye, Harbin 150001, People's Republic of China. Additional authors for this research include H.Y. Ge, X.L. Tang, S. Su, P. Tian, N. Xiao, H. Zhang, L. Zhang and P. Liu (see also Proteins).
Keywords for this news article include: Asia, Harbin, Proteins, Engineering, People's Republic of China
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