By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Biotechnology have been published. According to news originating from Beijing, People's Republic of China, by NewsRx correspondents, research stated, "Megalocytivirus is an important fish pathogen with a broad host range that includes turbot. In this study, proteomic analysis was conducted to examine turbot proteins modulated in expression by megalocytivirus infection."
Our news journalists obtained a quote from the research from the Chinese Academy of Science, "Thirty five proteins from spleen were identified to be differentially expressed at 2 days post-viral infection (dpi) and 7 dpi. Three upregulated proteins, i.e. heat shock protein 70 (Hsp70), Mx protein, and natural killer enhancing factor (NKEF), were further analyzed for potential antiviral effect. For this purpose, turbot were administered separately with the plasmids pHsp70, pMx, and pNKEF, which express Hsp70, Mx, and NKEF respectively, before megalocytivirus infection. Viral dissemination and propagation in spleen were subsequently determined. The results showed that the viral loads in fish administered with pNKEF were significantly reduced. To examine the potential of Hsp70, Mx, and NKEF as immunological adjuvant, turbot were immunized with a DNA vaccine in the presence of pHsp70, pMx, or pNKEF. Subsequent analysis showed that the presence of pNKEF and pHsp70, but not pMx, significantly reduced viral infection and enhanced fish survival. Taken together, these results indicate that NKEF exhibits antiviral property against megalocytivirus, and that both NKEF and Hsp70 may be used in DNA vaccine-based control of megalocytivirus infection. Biological significance This study provides the first proteomic picture of turbot in response to megalocytivirus infection. We demonstrated that megalocytivirus infection modulates the expression of turbot proteins associated with various cellular functions, and that one of the upregulated proteins, NKEF, exhibits antiviral effect when overexpressed in uiuo, while another upregulated protein, Hsp70, exhibits adjuvant effect when co-immunized with a DNA vaccine."
According to the news editors, the research concluded: "These results add molecular insights into turbot immune response induced by megalocytivirus and provide candidate proteins with application potentials in the control of megalocytivirus-associated disease."
For more information on this research see: Megalocytivirus-induced proteins of turbot (Scophthalmus maximus): Identification and antiviral potential. Journal of Proteomics, 2013;91():430-443. Journal of Proteomics can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Journal of Proteomics - www.elsevier.com/wps/product/cws_home/713351)
The news correspondents report that additional information may be obtained from J. Zhang, Chinese Academy Sci, Grad Univ, Beijing 100049, People's Republic of China. Additional authors for this research include Y.H. Hu, Z.Z. Xiao and L. Sun (see also technology.html">Biotechnology).
Keywords for this news article include: Asia, Biotechnology, Beijing, Peptides, Proteins, Amino Acids, DNA Vaccines, Synthetic Vaccines, People's Republic of China
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