By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Biotechnology. According to news reporting from Xinjiang, People's Republic of China, by NewsRx journalists, research stated, "It has been shown that cytokines can act as molecular adjuvant to enhance the immune response induced by DNA vaccines, but it is unknown whether interleukin 33 (IL-33) can enhance the immunocontraceptive effect induced by DNA vaccines. In the present study, we explored the effects of murine IL-33 on infertility induced by Lagurus lagurus zona pellucida 3 (Lzp3) contraceptive DNA vaccine administered by the mucosal route."
The news correspondents obtained a quote from the research from Xinjiang University, "Plasmid pcD-Lzp3 and plasmid pcD-mIL-33 were encapsulated with chitosan to generate the nanoparticle chi-(pcD-Lzp3+pcD-mIL-33) as the DNA vaccine. Sixty female ICR mice, divided into 5 groups (n=12/group), were intranasally immunized on days 0, 14, 28, and 42. After intranasal immunization, the anti-LZP3-specific IgG in serum and IgA in vaginal secretions and feces were determined by ELISA. The results showed that chi(pcD-Lzp3+pcD-mIL-33) co-immunization induced the highest levels of serum IgG, secreted mucosal IgA, and T cell proliferation. Importantly, mice co-immunized with chi-(pcD-Lzp3+pcD-mIL-33) had the lowest birth rate and mean litter size, which correlated with high levels of antibodies. Ovaries from infertile female mice co-immunized with chi-(pcD-Lzp3+pcD-mIL33) showed abnormal development of ovarian follicles, indicated by atretic follicles and loss of oocytes. Our results demonstrated that intranasal delivery of the molecular adjuvant mIL-33 with chi-pcD-Lzp3 significantly increased infertility by enhancing both systemic and mucosal immune responses."
According to the news reporters, the research concluded: "Therefore, chi-(pcD-Lzp3+pcD-mIL-33) co-immunization could be a strategy for controlling the population of wild animal pests."
For more information on this research see: Molecular adjuvant interleukin-33 enhances the antifertility effect of Lagurus lagurus zona pellucida 3 DNA vaccine administered by the mucosal route. Brazilian Journal of Medical and Biological Research, 2013;46(12):1064-1073. Brazilian Journal of Medical and Biological Research can be contacted at: Assoc Bras Divulg Cientifica, Faculdade Medicina, Sala 21, 14049 Ribeirao Preto, Sao Paulo, 00, Brazil (see also technology.html">Biotechnology).
Our news journalists report that additional information may be obtained by contacting Y.X. Tu, Xinjiang Univ, Xinjiang Key Lab Biol Resources & Genet Engn, Coll Life Sci & Technol, Urumqi 830046, Xinjiang, People's Republic of China. Additional authors for this research include X.P. Li, Z. Kadir and F.C. Zhang.
Keywords for this news article include: Asia, Ovum, Biotechnology, Xinjiang, Cytokines, DNA Research, DNA Vaccines, Interleukins, Zona Pellucida, Synthetic Vaccines, Extracellular Matrix, People's Republic of China
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