By a News Reporter-Staff News Editor at Biotech Week -- Fresh data on Apoptosis are presented in a new report. According to news reporting out of Shenyang, People's Republic of China, by NewsRx editors, research stated, "Neuroblastoma (NB) is the most common extracranial solid tumor in childhood. The present treatment including surgery, chemotherapy and radiation, which have only 40% long-term cure rates, and usually cause tumor recurrence."
Our news journalists obtained a quote from the research from China Medical University and Hospital, "Thus, looking for new effective and less toxic therapies has important significance. XAV939 is a small molecule inhibitor of tankyrase 1(TNKS1). The objective of this study is to investigate the effect of XAV939 on the proliferation and apoptosis of NB cell lines, and the related mechanism. In the present study, we used both XAV939 treatment and RNAi method to demonstrate that TNKS1 inhibition may be a potential mechanism to cure NB. MTT method was used for determining the cell viability and the appropriate concerntration for follow-up assays. The colony formation assay, Annexin V staining and cell cycle analysis were used for detecting colony forming ability, cell apoptosis and the percentage of different cell cycle. The Western blot was used for detecting the expression of key proteins of Wnt/ beta-catenin (Wnt/?-catenin) signaling pathway. The results showed that TNKS1 inhibition decreased the viability of SH-SY5Y, SK-N-SH and IMR-32 cells, induced apoptosis in SH-SY5Y as well as SK-N-SH cells, and led to the accumulation of NB cells in the S and G2/M phase of the cell cycle. Moreover, we demonstrated TNKS1 inhibition may in part blocked Wnt/?-catenin signaling and reduced the expression of anti-apoptosis protein. Finally, we also demonstrated that TNKS1 inhibition decreased colony formation in vitro."
According to the news editors, the research concluded: "These findings suggested that TNKS1 may be a potential molecule target for the treatment of NB."
For more information on this research see: XAV939, a tankyrase 1 inhibitior, promotes cell apoptosis in neuroblastoma cell lines by inhibiting Wnt/?-catenin signaling pathway. Journal of Experimental & Clinical Cancer Research, 2013;32(1):100. (BioMed Central - www.biomedcentral.com/; Journal of Experimental & Clinical Cancer Research - www.jeccr.com)
Our news journalists report that additional information may be obtained by contacting X.H. Tian, Dept. of Tissue Engineering, College of Basic Medical Sciences, China Medical University, Shenyang 110001, People's Taiwan. Additional authors for this research include W.J. Hou, Y. Fang, J. Fan, H. Tong, S.L. Bai, Q. Chen, H. Xu and Y. Li (see also Apoptosis).
Keywords for this news article include: Asia, Drugs, Shenyang, Apoptosis, Treatment, Tankyrases, Chemotherapy, Glycosyltransferases, Pentosyltransferases, People's Republic of China, Poly(ADP ribose) Polymerases.
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