By a News Reporter-Staff News Editor at Stem Cell Week -- Investigators publish new report on Stem Cell Research. According to news originating from Seattle, Washington, by NewsRx correspondents, research stated, "Myogenic progenitor cells derived from human embryonic stem cells (hESCs) can provide unlimited sources of cells in muscle regeneration but their clinical uses are largely hindered by the lack of efficient methods to induce differentiation of stem cells into myogenic cells. We present a novel approach to effectively enhance myogenic differentiation of human embryonic stem cells using aligned chitosan-polycaprolactone (C-PCL) nanofibers constructed to resemble the microenvironment of the native muscle extracellular matrix (ECM) in concert with Wnt3a protein."
Our news journalists obtained a quote from the research from the University of Washington, "The myogenic differentiation was assessed by cell morphology, gene activities, and protein expression. hESCs grown on C-PCL uniaxially aligned nanofibers in media containing Wnt3a displayed an elongated morphology uniformly aligned in the direction of fiber orientation, with increased expressions of marker genes and proteins associated with myogenic differentiation as compared to control substrates. The combination of Wnt3a signaling and aligned C-PCL nanofibers resulted in high percentages of myogenic-protein expressing cells over total treated hESCs (83% My5, 91% Myf6, 83% myogenin, and 63% MHC) after 2 days of cell culture. Significantly, this unprecedented high-level and fast myogenic differentiation of hESC was demonstrated in a culture medium containing no feeder cells."
According to the news editors, the research concluded: "This study suggests that chitosan-based aligned nanofibers combined with Wnt3a can potentially act as a model system for embryonic myogenesis and muscle regeneration."
For more information on this research see: Nanofiber-Based in Vitro System for High Myogenic Differentiation of Human Embryonic Stem Cells. Biomacromolecules, 2013;14(12):4207-4216. Biomacromolecules can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Biomacromolecules - www.pubs.acs.org/journal/bomaf6)
The news correspondents report that additional information may be obtained from M. Leung, University of Washington, Dept. of Pharmacol, Seattle, WA 98195, United States. Additional authors for this research include A. Cooper, S. Jana, C.T. Tsao, T.A. Petrie and M.Q. Zhang (see also Stem Cell Research).
Keywords for this news article include: Seattle, Washington, United States, Stem Cell Research, North and Central America
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