By a News Reporter-Staff News Editor at Biotech Week -- Investigators discuss new findings in Enzymes and Coenzymes. According to news reporting originating from Beijing, People's Republic of China, by NewsRx correspondents, research stated, "Herein, a double-stranded (ds) DNA microarray-based resonance light scattering (RLS) assay with multifunctional gold nanoparticle (GNP) probes has been developed for studying restriction endonuclease functionality and inhibition. Because of decreasing significantly melting temperature, the enzyme-cleaved dsDNAs easily unwind to form single-stranded (ss) DNAs."
Our news editors obtained a quote from the research from the Chinese Academy of Sciences, "The ssDNAs are hybridized with multiplex complementary ssDNAs functionalized GNP probes followed by silver enhancement and RLS detection. Three restriction endonucleases (EcoRI, BamHI and EcoRV) and three potential inhibitors (doxorubicin hydrochloride (DOX), ethidium bromide (EB) and an EcoRI-derived helical peptide (alpha 4)) were selected to demonstrate capability of the assay. Enzyme activities of restriction endonucleases are detected simultaneously with high specificity down to the limits of 2.0 x 10(-2) U/mL for EcoRI, 1.1 x 10(-2) U/mL for BamHI and 1.6 x 10(-2) U/mL for EcoRV, respectively."
According to the news editors, the research concluded: "More importantly, the inhibitory potencies of three inhibitors are showed quantitatively, indicating that our approach has great promise for high-throughput screening of restriction endonuclease inhibitors."
For more information on this research see: Assaying multiple restriction endonucleases functionalities and inhibitions on DNA microarray with multifunctional gold nanoparticle probes. Biosensors & Bioelectronics, 2014;52():118-123. Biosensors & Bioelectronics can be contacted at: Elsevier Advanced Technology, Oxford Fulfillment Centre The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England. (Elsevier - www.elsevier.com; Biosensors & Bioelectronics - www.elsevier.com/wps/product/cws_home/405913)
The news editors report that additional information may be obtained by contacting L. Ma, Univ Chinese Academy Sci, Beijing 100049, People's Republic of China. Additional authors for this research include Z.J. Zhu, T. Li and Z.X. Wang (see also Enzymes and Coenzymes).
Keywords for this news article include: Asia, Beijing, Esterases, DNA Research, Endonucleases, Nanotechnology, Gold Nanoparticles, Emerging Technologies, Enzymes and Coenzymes, People's Republic of China
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