By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators discuss new findings in Carrier Proteins. According to news reporting originating in Kongens Lyngby, Denmark, by NewsRx journalists, research stated, "In this paper, we demonstrate recordings of the ion channel activity across the cell membrane in a biological cell by employing the so-called patch clamping technique on an injection-molded polymer microfluidic device. The findings will allow direct recordings of ion channel activity to be made using the cheapest materials and production platform to date and with the potential for very high throughput."
The news reporters obtained a quote from the research from the Technical University of Denmark, "The employment of cornered apertures for cell capture allowed the fabrication of devices without through holes and via a scheme comprising master origination by dry etching in a silicon substrate, electroplating in nickel and injection molding of the final part. The most critical device parameters were identified as the length of the patching capillary and the very low surface roughness on the inside of the capillary. The cross-sectional shape of the orifice was found to be less critical, as both rectangular and semicircular profiles seemed to have almost the same ability to form tight seals with cells with negligible leak currents. The devices were functionally tested using human embryonic kidney cells expressing voltage-gated sodium channels (Nav1.7) and benchmarked against a commercial state-of-the-art system for automated ion channel recordings. These experiments considered current-voltage (IV) relationships for activation and inactivation of the Nav1.7 channels and their sensitivity to a local anesthetic, lidocaine."
According to the news reporters, the research concluded: "Both IVs and lidocaine dose-response curves obtained from the injection-molded polymer device were in good agreement with data obtained from the commercial system."
For more information on this research see: Ion channel recordings on an injection-molded polymer chip. Lab On a Chip - Miniaturisation for Chemistry and Biology, 2013;13(24):4784-93 (see also Carrier Proteins).
Our news correspondents report that additional information may be obtained by contacting S. Tanzi, Dept. of Micro- and Nanotechnology, Technical University of Denmark, Building 345E, DK-2800 Kongens Lyngby, Denmark. Additional authors for this research include M. Matteucci, T.L. Christiansen, S. Friis, M.T. Christensen, J. Garnaes, S. Wilson, J. Kutchinsky and R. Taboryski.
Keywords for this news article include: Europe, Denmark, Ion Channels, Kongens Lyngby, Carrier Proteins, Membrane Glycoproteins, Membrane Transport Proteins.
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