By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Life Science Research. According to news reporting originating from Konstanz, Germany, by NewsRx correspondents, research stated, "Optical microscopy is an indispensable tool that is driving progress in cell biology. It still is the only practical means of obtaining spatial and temporal resolution within living cells and tissues."
Our news editors obtained a quote from the research from the University of Konstanz, "Most prominently, fluorescence microscopy based on dye-labeling or protein fusions with fluorescent tags is a highly sensitive and specific method of visualizing biomolecules within sub-cellular structures. It is however severely limited by labeling artifacts, photo-bleaching and cytotoxicity of the labels. Coherent Raman Scattering (CRS) has emerged in the last decade as a new multiphoton microscopy technique suited for imaging unlabeled living cells in real time with high three-dimensional spatial resolution and chemical specificity. This technique has proven to be particularly successful in imaging unstained lipids from artificial membrane model systems, to living cells and tissues to whole organisms. In this article, we will review the experimental implementations of CRS microscopy and their application to imaging lipids. We will cover the theoretical background of linear and non-linear vibrational micro-spectroscopy necessary for the understanding of CRS microscopy. The different experimental implementations of CRS will be compared in terms of sensitivity limits and excitation and detection methods."
According to the news editors, the research concluded: "Finally, we will provide an overview of the applications of CRS microscopy to lipid biology."
For more information on this research see: Nonlinear vibrational microscopy applied to lipid biology. Progress In Lipid Research, 2013;52(4):615-32. (Elsevier - www.elsevier.com; Progress In Lipid Research - www.elsevier.com/wps/product/cws_home/407)
The news editors report that additional information may be obtained by contacting A. Zumbusch, Dept. of Chemistry, University of Konstanz, 78457 Konstanz, Germany. Additional authors for this research include W. Langbein and P. Borri (see also Life Science Research).
Keywords for this news article include: Europe, Germany, Konstanz, Life Science Research.
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