By a News Reporter-Staff News Editor at Health & Medicine Week -- New research on Health and Medicine is the subject of a report. According to news reporting from Tifton, Georgia, by NewsRx journalists, research stated, "Apomixis is a naturally occurring asexual mode of seed reproduction resulting in offspring genetically identical to the maternal plant. Identifying differential gene expression patterns between apomictic and sexual plants is valuable to help deconstruct the trait."
The news correspondents obtained a quote from the research from the University of Georgia, "Quantitative RT-PCR (qRT-PCR) is a popular method for analyzing gene expression. Normalizing gene expression data using proper reference genes which show stable expression under investigated conditions is critical in qRT-PCR analysis. We used qRT-PCR to validate expression and stability of six potential reference genes (EF1alpha, EIF4A, UBCE, GAPDH, ACT2 and TUBA) in vegetative and reproductive tissues of B-2S and B-12-9 accessions of C. ciliaris. Among tissue types evaluated, EF1alpha showed the highest level of expression while TUBA showed the lowest. When all tissue types were evaluated and compared between genotypes, EIF4A was the most stable reference gene. Gene expression stability for specific ovary stages of B-2S and B-12-9 was also determined. Except for TUBA, all other tested reference genes could be used for any stage-specific ovary tissue normalization, irrespective of the mode of reproduction. Our gene expression stability assay using six reference genes, in sexual and apomictic accessions of C. ciliaris, suggests that EIF4A is the most stable gene across all tissue types analyzed. All other tested reference genes, with the exception of TUBA, could be used for gene expression comparison studies between sexual and apomictic ovaries over multiple developmental stages."
According to the news reporters, the research concluded: "This reference gene validation data in C. ciliaris will serve as an important base for future apomixis-related transcriptome data validation."
For more information on this research see: Selection and validation of reference genes for gene expression analysis in apomictic and sexual Cenchrus ciliaris. Bmc Research Notes, 2013;6():397. (BioMed Central - www.biomedcentral.com/; Bmc Research Notes - www.biomedcentral.com/bmcresnotes/)
Our news journalists report that additional information may be obtained by contacting B. Simon, Dept. of Horticulture, The University of Georgia Tifton Campus, Tifton, GA 31793, United States. Additional authors for this research include J.A. Conner and P. Ozias-Akins (see also Health and Medicine).
Keywords for this news article include: Tifton, Georgia, United States, Machine Learning, Health and Medicine, Emerging Technologies, Gene Expression Analysis, North and Central America.
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